TMS5基因在小麦BNS不育系育性转换中的差异表达分析  

Gene Differential Expression Analysis of TMS5 in the Fertility Conversion of Wheat BNS Sterile Line

作　　者：刘书含 陈磊[1] 张建朝 胡甘 孙君艳[1] 刘东涛 王军卫[2] Liu Shuhan;Chen Lei;Zhang Jianchao;Hu Gan;Sun Junyan;Liu Dongtao;Wang Junwei(School of Agriculture,Xinyang Agriculture and Forestry University,Xingyang 464000,Henan,China;College of Agronomy,Northwest A&F University/National Yangling Agricultural Biotechnology&Breeding Center/Yangling Branch of State Wheat Improvement Center/Wheat Breeding Engineering Research Center,Ministry of Education/Key Laboratory of Crop Heterosis of Shaanxi Province,Yangling 712100,Shaanxi,China;Xuzhou Institute of Agricultural Science,Xuhuai District,Jiangsu Province,Xuzhou 221131,Jiangsu,China)

机构地区：[1]信阳农林学院农学院,河南信阳464000 [2]西北农林科技大学农学院/国家杨凌农业生物技术育种中心/国家小麦改良中心杨凌分中心/小麦育种教育部工程研究中心/陕西省作物杂种优势研究与利用重点实验室,陕西杨凌712100 [3]江苏徐淮地区徐州农业科学研究所,江苏徐州221131

出　　处：《作物杂志》2023年第5期24-29,共6页Crops

基　　金：信阳市小麦栽培与生理生态重点实验室;河南省科技攻关项目(212102110049);陕西省作物杂种优势研究与利用重点实验室后补助项目(No.2018SZS-22);徐州市重点研发(现代农业)计划项目(KC20036)。

摘　　要：TMS5基因编码RNase Z蛋白,它是水稻中报道的一个温敏核不育基因。分析比较小麦中TMS5同源基因在小麦BNS不育系和可育系花药不同发育时期表达的差异,为揭示小麦BNS温敏核雄性不育的机制提供理论依据。以小麦BNS不育系和可育系花药为试验材料,克隆获得408bp的小麦TMS5同源序列,其与水稻TMS5基因序列一致性达80%。定量分析小麦TMS5在花药发育的四分体期、单核早期、单核晚期、二核期和三核期的表达发现,该基因主要在四分体期表达,并且其在可育系花药中的表达量要远高于不育系。小麦TMS5基因与BNS温敏核雄性不育系的花粉败育密切相关,且四分体期是其调控的关键时期。The TMS5 is a thermosensitive sterility gene of rice,which encodes a RNase Z protein.We analyzed the expression differences of wheat TMS5 gene in wheat BNS sterile line and fertile line at different developmental stages of anthers,aiming to provide a theoretical basis for revealing the mechanism of wheat BNS thermosensitive sterility.Using the sterile and fertile anthers of wheat BNS as test materials,a 408bp sequence of wheat TMS5 was cloned,which was 80%identical to the sequence of the TMS5 in rice.Compared the TMS5 expression in stages of wheat anther development including tetrad,early mononuclear,late mononuclear,dinuclear and trinuclear,the results showed that the TMS5 was mainly expressed in the tetrad stage,and the TMS5 expression level in anthers of fertile lines was much higher than that of sterile lines.The wheat TMS5 gene was closely related to the pollen abortion of wheat BNS thermosensitive sterile line,and the tetrad stage was the key period of TMS5’s regulation.

关 键 词：小麦 温敏核雄性不育 BNS TMS5基因 荧光实时定量PCR 

分 类 号：S512.1[农业科学—作物学]