Qinghua decoction(清化饮)improves chronic nonbacterial prostatitis possibly via regulating the chromogranin A/nerve growth factor/tyrosine kinase A signaling pathway mediated by inflammatory factors  

作　　者：HAN Yunpeng YU Wentao ZHANG Ying XU Huazhou DENG Guoxing FANG Chaoyi 

机构地区：[1]School of Basic Medical Sciences,Hebei University of Chinese Medicine,Shijiazhuang 050200,China [2]Hebei Key Laboratory of Integrated Chinese and Western Medicine for Lung Disease Research,Shijiazhuang 050091,China

出　　处：《Journal of Traditional Chinese Medicine》2023年第4期695-703,共9页中医杂志（英文版）

基　　金：Hebei Administration of Traditional Chinese Medicine Project:Study on the Neuroendocrine Mechanism of Chronic Prostatitis Rats Regulated by the Method of Clearing heat and Dampness,Activating Blood and Removing Stasis(No.2019087);Hebei Provincial Department of Education for Postgraduate Innovation Ability Training Project:the Mechanism of Improving Pulmonary Function of COPD Rats with Lung Qi Deficiency by Regulating Intestinal Bacteria with Peitu Shengjin Formula(No.CXZZBS2020151);Provincial Universities Basic Research Funds Special Project:Study on the Mechanism of Shenling Baizhu Powder Based on Lung Intestine Axis to Interfere with the Deficiency of Lung and Spleen in Experimental COPD(YJZ2019010)。

摘　　要：OBJECTIVE:To explore the mechanism by which Qinghua decoction(清化饮)regulates neuroendocrine inflammation in chronic nonbacterial prostatitis(CNP)model rats and provide an experimental basis for clinical treatment.METHODS:The rats were randomly divided into six groups:normal control,model,Qianlie Tongyu capsule,low-dose Qinghua decoction,medium-dose Qinghua decoction,and high-dose Qinghua decoction group with six rats in each group.Rats in each group were sacrificed on the 29th day of treatment,and blood and prostate tissues were collected.Serum levels of tumor necrosis factor-alpha and interleukins 1-beta,6,8,and 10(TNF-αand IL-1β,-6,-8,and-10,respectively)were measured using enzyme-linked immunosorbent assay.The pathological changes in the rat prostate tissue in each group were observed under a light microscope.The expression levels of chromogranin A(CgA),nerve growth factor(NGF),and tyrosine kinase A(TrkA)were detected using reverse transcription quantitative polymerase chain reaction.Western blotting was used to detect protein expression of CgA,NGF,and TrkA.RESULTS:In the model group,the prostate capsule membrane and stroma were significantly dilated with more inflammatory cells infiltrating the stroma and perivessels.TNF-α,IL-1β,-6,and-8,CgA,NGF,and TrkA levels increased,whereas the content of IL-10 decreased,which was statistically significant compared to that in the normal control group(P<0.05).Prostate tissue cells in the high-dose group were neatly arranged with no obvious inflammatory cell infiltration.When compared with the model group,the high-dose Qinghua decoction group showed a significant improvement in these indices(P<0.05).CONCLUSION:Qinghua decoction led to inhibition of pathological changes in the prostate tissue of rats with CNP,regulation of inflammatory cytokine expression,and inhibition in the expression of CgA,NGF,and TrkA.This mechanism may be primarily related to regulation of the CgA/NGF/TrkA signaling pathway mediated by various inflammatory factors.

关 键 词：inflammation INTERLEUKINS chromogranin A nerve growth factor chronic nonbacterial prostatitis Qinghua decoction 

分 类 号：R285.5[医药卫生—中药学]