Efficacy of electroacupuncture stimulating Shenmen(HT7),Baihui(GV20),Sanyinjiao(SP6)on spatial learning and memory deficits in rats with insomnia induced by para-chlorophenylalanine:a single acupoint vs combined acupoints  

作　　者：QIAO Lina SHI Yinan TAN Lianhong JIANG Yanshu YANG Yongsheng 

机构地区：[1]Department of Biochemistry and Molecular Biology,Institute of Acupuncture and Moxibustion,China Academy of Chinese Medical Sciences,Beijing100700,China [2]College of Acupuncture-moxibustion and Massage,Changchun University of Traditional Chinese Medicine,Changchun 130117,China

出　　处：《Journal of Traditional Chinese Medicine》2023年第4期704-714,共11页中医杂志（英文版）

基　　金：“973”Program of the Ministry of Science and Technology of China:Research on Acupoint Optimization,Combination and Evaluation Methods(No.2014CB543103);the SelfSelected Research Program from of China Academy of Chinese Medical Sciences:Study on the Mechanism of Central Amygdala Nucleus Mediated Electroacupuncture on Relieving Chronic Pain and Related Aversive Mood(No.ZZ13-YQ-063)。

摘　　要：OBJECTIVE:To investiage the effect of electroacupuncture(EA)at a single acupoint of Shenmen(HT7),Baihui(GV20),Sanyinjiao(SP6)and at combined acupoints of Shenmen(HT7)and Baihui(GV20)and Sanyinjiao(SP6)on the PKA/CREB and BDNF/TrkB signaling,as well as neuroapoptosis and neurogenesis in hippocampus and elucidate the underlying mechanism of single and combined acupoints on ameliorating spatial learning and memory deficits in a rat model of primary insomnia.METHODS:Primary insomnia was modeled by intraperitoneal injection of para-chlorophenylalanine(PCPA)once daily for 2 d.EA was applied at Shenmen(HT7),Baihui(GV20),Sanyinjiao(SP6),or Shenmen(HT7)+Baihui(GV20)+Sanyinjiao(SP6)(combined)for 30 min daily for 4 d.Spatial learning and memory function was evaluated by the Morris water maze(MWM)test.Protein expressions of hippocampal cAMP-dependent protein kinase(PKA)-Cβ,phosphorylated cAMP-responsive element-binding protein(p-CREB),brainderived neurotrophic factor(BDNF),and tyrosine kinase receptor B(TrkB)were evaluated by Western blotting.Neuronal apoptosis in the hippocampus was detected with the transferase-mediated dUTP-X nick end labeling assay.Endogenous neurogenesis was examined with bromodeoxyuridine staining.The MWM test and hippocampal p-CREB,BDNF,and TrkB protein levels in the combined acupoints group were evaluated after the administration of a PKA-selective inhibitor(H89).RESULTS:Spatial learning and memory were significantly impaired in rats with insomnia.The spatial learning deficits were ameliorated in the Shenmen(HT7),Baihui(GV20),Sanyinjiao(SP6),and combined groups;this improvement was significantly greater in the combined group than the single acupoint groups.The spatial memory impairment was improved in the combined,Baihui(GV20),and Shenmen(HT7)groups,but not the Sanyinjiao(SP6)group.The expressions of PKA-Cβ,p-CREB,BDNF,and TrkB were decreased in rats with insomnia.All these proteins were significantly upregulated in the combined group.PKA/p-CREB protein levels were elevated in the Baihui(GV20)and S

关 键 词：sleep initiation and maintenance disorders learning memory hippocampus neuronal plasticity acupoints combination 

分 类 号：R245.97[医药卫生—针灸推拿学]