羟基含量对Ru(bpy)_(3)^(2+)/CDs@H_(2)O_(2)体系检测多巴胺的影响  

Effect of Hydroxyl Content on Dopamine Detection by Ru(bpy)_(3)^(2+)/CDs@H_(2)O_(2) System

作　　者：马纳 秦莉莉 刘锋净 齐宝平 尚冰冰 MA Na;QIN Li;LIU Fengjing;QI Baoping;SHANG Bingbing(Hubei Key Laboratory of Biologic Resources Protection and Utilization,Hubei Minzu University,Enshi 445000,Hubei,China;School of Chemistry and Environmental Engineering,Hubei Minzu University,Enshi 445000,Hubei,China)

机构地区：[1]湖北民族大学生物资源保护与利用湖北省重点实验室,湖北恩施445000 [2]湖北民族大学化学与环境工程学院,湖北恩施445000

出　　处：《武汉大学学报（理学版）》2023年第3期349-355,共7页Journal of Wuhan University:Natural Science Edition

基　　金：国家自然科学基金(22264014);湖北省教育厅科学技术研究项目(B2021157);生物资源保护与利用湖北省重点实验室开放基金(PT012106);湖北民族大学高水平科研成果校内培育项目(PY22006);湖北民族大学博士启动基金(MY2015B019)。

摘　　要：以2B铅笔芯为原料,用电氧化法在水相中制备荧光碳点(CDs),再通过H_(2)O_(2)氧化得到CDs@H_(2)O_(2),利用透射电子显微镜(TEM)、荧光光谱、Fourier变换红外光谱(FT-IR)与X射线光电子能谱(XPS)对CDs和CDs@H_(2)O_(2)进行表征。结果表明,相对于CDs,CDs@H_(2)O_(2)表面具有更丰富的羟基官能团。将CDs与CDs@H_(2)O_(2)分别用作Ru(bpy)_(3)^(2+)阳极电化学发光(ECL)的共反应剂,Ru(bpy)_(3)^(2+)/CDs@H_(2)O_(2)体系的ECL信号强度是Ru(bpy)_(3)^(2+)/CDs体系的8.2倍,证实羟基是CDs作Ru(bpy)_(3)^(2+)共反应剂的活性官能团。基于多巴胺(DA)对Ru(bpy)_(3)^(2+)/CDs@H_(2)O_(2)体系ECL信号的猝灭作用,构建了关于DA的ECL猝灭传感器,检测的线性范围为1.6~56.0μmol/L,检出限为0.23μmol/L(S/N=3),且实现了对人血清中DA浓度的检测。Using 2B pencil lead as raw material,fluorescent carbon dots (CDs) was prepared in aqueous phase by electric oxidation method,and then CDs@H_(2)O_(2) was obtained by H_(2)O_(2) oxidation.CDs and CDs@H_(2)O_(2) were characterized by electron transmission electron microscopy (TEM),fluorescence spectroscopy,Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy(XPS).The results show that the surface of CDs@H_(2)O_(2) has more hydroxyl functional groups than CDs. CDs and CDs@H_(2)O_(2) were respectively used as co-reactant of Ru(bpy)_(3)^(2+)anode electrochemiluminescence (ECL).The ECL signal of Ru(bpy)_(3)^(2+)/CDs@H_(2)O_(2) system was 8.2 times that of Ru(bpy)_(3)^(2+)/CDs system.It was confirmed that hydroxyl group was the active functional group of CDs as Ru(bpy)_(3)^(2+)coreactive agent.Based on the quenching effect of dopamine (DA) on ECL signal in Ru(bpy)_(3)^(2+)/CDs@H_(2)O_(2) system,the ECL quenching sensor for DA was constructed.The linear range of detection was 1.6-56.0μmol/L,and the detection limit was 0.23μmol/L (S/N=3).The detection of DA concentration in human serum was realized.

关 键 词：碳点 多巴胺 电致化学发光 共反应剂 联吡啶钌 血清 

分 类 号：O657.1[理学—分析化学]