Pingxiao capsule(平消胶囊)inhibits lung metastasis of triple-negative breast cancer and sensitizes breast cancer to radiotherapy  

作　　者：TANG Wenjuan HU Yu ZHU Man DONG Mingzhi LIU Tieming SARWAR Ammar ZHAN Yingzhuan ZHANG Yanmin 

机构地区：[1]School of Pharmacy,Health Science Center,Xi’an Jiaotong University,Xi’an 710061,China [2]State Key Laboratory of Shaanxi for Natural Medicines Research and Engineering,Xi'an 710061,China [3]Xi’an C.P.Pharmaceutical Co.,Ltd.,Xi’an,710043,China

出　　处：《Journal of Traditional Chinese Medicine》2023年第5期897-905,共9页中医杂志（英文版）

基　　金：Natural Science Basic Research Plan of Shaanxi Province in China:Chloroguanil Inhibits Breast Cancer Progression by Targeting GHRH/GHRHR Pathway(No.2021JM-030);the Project from the Basic Research Project in Shaanxi Administration of Traditional Chinese Medicine:Inhibitory Effects of Xiakucao(Spica Prunellae Vulgaris)-Maozhuacao(Radix Ranunculi Ternati)Drug Pair on Breast Cancer(No.JCMS028)。

摘　　要：OBJECTIVES: To investigate the anticancer effect of Pingxiao capsule(平消胶囊, PXC) on the treatment of breast cancer in vitro and in vivo. METHODS: The inhibition of PXC on cell viability and proliferation was determined by cell counting kit-8, EdU assay and colony formation assay, respectively. The effect of PXC on cell apoptosis was detected by using flow cytometry. The suppression of PXC on cell migration and invasion was investigated by chamber assay. To investigate the underlying molecular mechanisms, the expression of proteins related to epithelial to mesenchymal transition(EMT) was analyzed by Western blotting in breast cancer cells and by immunohistochemistry in tumor tissues. The anticancer effect of PXC in vivo was evaluated by using MDA-MB-231 xenograft model and 4T1 metastatic breast cancer model. RESULTS: Our results indicated that triple-negative breast cancer(TNBC) cell lines MDA-MB-231 and MDAMB-468 were sensitive to PXC. PXC potently inhibited the proliferation, colony formation, migration, and invasion of MDA-MB-231 and MDA-MB-468 cells in vitro. Then, in vivo MDA-MB-231 xenograft model depicted that PXC significantly reduced tumor size and weight compared with Control. 4T1 lung metastasis model showed that PXC significantly inhibited breast cancer cell spreading to lungs in mice. Mechanistically, PXC inhibited EMT process by reducing cadherin turnover in TNBC. Furthermore, PXC in combination with 8 Gy X-ray treatment obviously promoted the induction of apoptosis, and suppressed cell proliferation. CONCLUSION: PXC could inhibit the proliferation and invasion of TNBC both in vitro and in vivo, and exerted its anti-metastatic effect by regulating cadherin turnover, Furthermore, it sensitized the TNBC cells to radiotherapy. The data supported further development of PXC as an adjuvant-therapy agent for TNBC.

关 键 词：triple negative breast neoplasms anti-cancer activity Pingxiao capsule 

分 类 号：R285[医药卫生—中药学]