Electroacupuncture stimulating Zhongji(CV3),Guanyuan(CV4),and bilateral Dahe(KI12)attenuates inflammation in rats with chronic nonbacterial prostatitis induced by estradiol through inhibiting toll-like receptor 4 pathway  

作　　者：LI Zhihao HAN Wenjun SONG Xiuling LI Yan CHEN Yuelai 

机构地区：[1]Department of Acupuncture-Moxibustion,Tuina and Traumatology,Shanghai Sixth People's Hospital Affiliated to Shanghai Jiaotong University Shool of Medicine,Shanghai 200233,China [2]Sleep Medical Center,Longhua Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China [3]Department of Andrology,the Seventh People's Hospital affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200137,China [4]Graduate School of Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China

出　　处：《Journal of Traditional Chinese Medicine》2023年第5期963-972,共10页中医杂志（英文版）

基　　金：the Program of Shanghai Pudong New Area Famous Traditional Chinese Medicine(PWRzm2020-01)。

摘　　要：OBJECTIVE:To investigate the anti-inflammatory effect of electroacupuncture(EA)on chronic prostatitis/chronic pelvic pain syndrome(CP/CPPS),also known as chronic nonbacterial prostatitis(CNP),and explore its underlying mechanism.METHODS:A CNP rat was established by surgical castration combined with 17-βestradiol injection in male Sprague-Dawley rats for thirty consecutive days.The CNP rats received EA treatment once a day for eight days.Chronic pelvic pain was evaluated by mechanical withdrawal threshold measurement.The histological change was assessed by hematoxylin-eosin staining.The inflammatory cytokines in prostates were determined by enzyme-linked immunosorbent assays.The expressions of toll-like receptor 4(TLR4),myeloid differentiation factor 88(MyD88),inhibitors of kappa-B alpha(IκBα),and nuclear factor-kappa B(NF-κB)were detected by Western blotting.The nuclear translocation of NF-κB and the location of TLR4 were observed with immunofluorescence staining.RESULTS:The results showed that EA decreased the prostate index,upregulated the mechanical withdrawal threshold,restored the histomorphology of the prostate,reduced the inflammatory factor levels,inhibited NF-κB p65 nuclear translocation,and downregulated the expression levels of critical proteins involved in the TLR4/NF-κB signaling pathway in prostates.CONCLUSIONS:Our findings suggested that EA could relieve pelvic pain and attenuate prostatic inflammation in estradiol-induced CNP rats.The underlying mechanism may be related to the inhibition of the TLR4/NF-κB signaling pathway.

关 键 词：chronic nonbacterial prostatitis ELECTROACUPUNCTURE morphology anti-inflammatory agents toll-like receptors 

分 类 号：R245[医药卫生—针灸推拿学]