鸦葱疱疹膏长期毒性实验研究  

Experimental study on long-term toxicity test of Scorzonera Herpes Zoster Ointment

作　　者：皇甫佳欣 初霞 秦臻 沈德新 HUANGFU Jiaxin;CHU Xia;QIN Zhen;SHEN Dexin(Department of Pediatrics,No.988 Hospital of Logistic Support Forces of the People’s Liberation Army,Henan Province,Zhengzhou450042,China;Department of Pathology,No.988 Hospital of Logistic Support Forces of the People’s Liberation Army,Henan Province,Zhengzhou450042,China)

机构地区：[1]中国人民解放军联勤保障部队第九八八医院儿科,河南郑州450042 [2]中国人民解放军联勤保障部队第九八八医院病理科,河南郑州450042

出　　处：《中国医药导报》2023年第25期32-37,共6页China Medical Herald

基　　金：河南省医学科技攻关计划联合共建项目(LHGJ20190879)。

摘　　要：目的观察鸦葱疱疹膏对新西兰兔的长期毒性。方法选用健康清洁级10~14周龄,体重2.0~2.5 kg新西兰兔32只,雌雄各半,按性别及随机数字表法分为对照组、鸦葱疱疹膏高剂量组(2 g生药/kg)、鸦葱疱疹膏中剂量组(1 g生药/kg)、鸦葱疱疹膏低剂量组(0.5 g生药/kg),每组8只。四组均对称脱去脊柱两侧毛,共150 cm^(2)。各组每周经皮给药6 d,连续用药4周,观察记录一般情况。给药4周后,每组取4只新西兰兔检测血液学指标、血液生化指标;然后解剖新西兰兔,取其脏器称重,计算脏器指数;并对脏器及给药局部皮肤进行组织病理学检查。剩余动物停药饲养恢复2周后,检测上述各项指标。结果给药4周后及停药恢复2周后,四组未发现明显红斑、水肿等刺激性反应;四组一般情况良好,饮水、摄食未见异常,皮毛光泽,活动行为及二便均未见异常;体重均逐渐增加,生长发育良好。鸦葱疱疹膏高、中、低剂量组均未见因药物中毒引起的动物死亡或其他毒副作用。给药4周后及停药2周后,四组白细胞计数、中性粒细胞比率、淋巴细胞比率、单核细胞比率、红细胞计数、血红蛋白、红细胞压积、血小板计数、凝血酶原时间、凝血酶时间,谷丙转氨酶、谷草转氨酶、总蛋白、白蛋白、总胆红素、碱性磷酸酶、钾、钠、氯、尿素氮、肌酐、肌酸激酶、血糖,肝脏、心脏、脾、肺、胰腺、肾脏系数比较,差异无统计学意义(P>0.05)。给药4周后及停药2周后,四组脏器形态、硬度、色泽均未见药物引起异常;各组心脏、肝脏、脾、肺、肾脏、肾上腺、胰腺、脑、胃、睾丸、小肠、直肠、膀胱、胸腺病理组织形态学检查均无异常组织学改变,皮肤病理组织形态学检查均未见水肿、表皮增生、角化等现象。结论鸦葱疱疹膏长期用药对新西兰兔无明显毒性作用。Objective To observe the long-term toxicity test of Scorzonera Herpes Zoster Ointment on New Zealand rabbits.Methods A total of 32 healthy and specific pathogen free New Zealand rabbits aged 10-14 weeks and weighing 2.0-2.5 kg,half male and half female were selected,and they were divided into control group,Scorzonera Herpes Zoster Ointment high dose group(2 g crude drug/kg),Scorzonera Herpes Zoster Ointment middle dose group(1 g crude drug/kg),and Scorzonera Herpes Zoster Ointment low dose group(0.5 g crude drug/kg),with eight rabbits with each group.The hairs on both sides of the spine were symmetrically removed in four groups,with a total of 150 cm^(2).Each group was given percutaneous administration for six days a week for four weeks,and the general situation was observed and recorded.After four weeks of administration,four New Zealand rabbits were selected from each group to detect hematological indexes and blood biochemical indexes;then the New Zealand rabbit was dissected,its organs were weighed,and the organ index was calculated;histopathological examination of the organs and the local skin were performed.The remaining animals were fed and recovered for two weeks after drug withdrawal,and the above indexes were detected.Results Four weeks after administration and two weeks after withdrawal,no obvious erythema,edema and other irritant reactions were found in four groups;the general condition of four groups was good,no abnormalities were found in drinking water,feeding,fur luster,activity,and bowel movements.The body weight increased gradually and the growth was good.There were no death or other toxic side effects caused by Scorzonera Herpes Zoster Ointment high,medium,and low dose groups.Four weeks after administration and two weeks after withdraw al,there were no significant differences in white blood cell count,neutrophil ratio,lymphocyte ratio,monocyte ratio,red blood cell count,hemoglobin,hematocrit,platelet count,prothrombin time,thrombin time,glutamic-pyruvic transaminase,glutamic-oxalacetic transamin

关 键 词：鸦葱 鸦葱疱疹膏 长期毒性实验 经皮给药 新西兰兔 

分 类 号：R75[医药卫生—皮肤病学与性病学]