平眩方对椎动脉型颈椎病模型SD大鼠的治疗作用及机制研究  

Study on the therapeutic effect and mechanism of Pingxuan Formula on cervical spondylotic arteriopathy model SD rats

作　　者：王怡璇 彭楠 肖晶晶 李腾龙[1] 李璐 WANG Yixuan;PENG Nan;XIAO Jingjing;LI Tenglong;LI Lu(The First Department of Orthopedics,the Second Affiliated Hospital of Hunan University of Chinese Medicine,Hunan Province,Changsha 410005,China;Department of Orthopaedics and Traumatology Traditional Chinese Medicine,the First People’s Hospital of Shuangliu District,Sichuan Province,Chengdu 610200,China)

机构地区：[1]湖南中医药大学第二附属医院骨伤一科,湖南长沙410005 [2]成都市双流区第一人民医院中医骨伤科,四川成都610200

出　　处：《中国医药导报》2023年第26期33-37,共5页China Medical Herald

基　　金：湖南省教育厅科学研究项目(19C1409);湖南中医药大学科研项目计划任务项目(2022XYLH040)。

摘　　要：目的探讨平眩方对椎动脉型颈椎病大鼠模型血液流变学、血浆内皮素(ET)及血清一氧化氮(NO)表达水平、椎动脉血管壁中膜厚度的影响及作用机制。方法将60只SPF级SD大鼠按照随机数字表法分为正常组,模型组,氟桂利嗪组,平眩方低、中、高剂量组,各10只。除正常组外,其余各组均采用改良硬化剂造模法建立椎动脉型颈椎病大鼠模型。造模4周后,平眩方低、中、高剂量组每日分别灌胃平眩方浓缩液7.8、15.6、31.2 g/kg,氟桂利嗪组每日灌胃盐酸氟桂利嗪胶囊0.9 mg/kg,正常组和模型组大鼠不予治疗。药物治疗2周后,测定各组大鼠椎动脉血流情况、血液流变学指标(低、中、高切变率下全血黏度,全血高/低切相对指数,红细胞聚集指数)血浆ET及血清NO水平、椎动脉血管壁中膜厚度。结果与正常组比较,模型组大鼠椎动脉平均血流速度降低、搏动指数及阻力指数升高,血液流变学各项指标均升高,血浆ET含量升高,血浆NO含量降低,椎动脉血管壁中膜厚度增加,差异有统计学意义(P<0.05)。与模型组比较,平眩方中、高剂量组大鼠椎动脉平均血流速度升高、搏动指数及阻力指数升高,血液流变学各项指标均降低,血浆ET含量降低,血浆NO含量增加,椎动脉血管壁中膜厚度降低,差异有统计学意义(P<0.05)。结论平眩方能有效改善椎动脉型颈椎病模型大鼠椎动脉血流情况及血液黏度,能够缓解血管痉挛、抑制血管平滑肌细胞增殖,这可能是平眩方治疗大鼠椎动脉型颈椎病的作用机制之一。Objective To investigate the effects of Pingxuan Formula on hemorheology,plasma endothelin(ET),and serum nitric oxide(NO)expression levels,and the medial membrane thickness of the vertebral artery wall in the rat model of vertebral arterial cervical spondylosis.Methods According to random number table method,60 SPF SD rats were divided into normal group,model group,Flunarizine group,Pingxuan Formula low,medium,and high dose groups,with ten rats in each group.In addition to the normal group,the rats with vertebral artery type cervical spondylosis were established by modified hardener molding method.After four weeks of modeling,the Pingxun Formula low,medium,and high dose groups were given oral administration of concentrated liquid of Pingxun Formula 7.8,15.6 and 31.2 g/kg,respectively,and the Fluunarizine group was given oral administration of Fluunarizine Hydrochloride Capsules 0.9 mg/kg,respectively.The normal group and model group were not treated.After two weeks of drug treatment,the blood flow of the vertebral artery,hemorheology indexes(whole blood viscosity,plasma viscosity,whole blood low/high shear relative index,red blood cell aggregation index),plasma ET,and serum NO levels,and the medial membrane thickness of the vertebral artery wall were measured in each group.Results Compared with the normal group,the average blood flow velocity of the vertebral artery in the model group was decreased,the pulsation index and resistance index of vertebral were increased,and the indexes of hemorheology were increased,the plasma ET content was increased,the plasma NO content was decreased,and the middle membrane thickness of the vertebral artery wall was increased,with statistical significance(P<0.05).Compared with model group,the average blood flow velocity,pulse index and resistance index of vertebral artery were increased,the indexes of hemorheology were decreased,the plasma ET content was decreased,the plasma NO content was increased,and the middle membrane thickness of vertebral artery wall was decreased,with stat

关 键 词：椎动脉型颈椎病 平眩方 血液流变学 血浆内皮素 一氧化氮 

分 类 号：R681[医药卫生—骨科学]