氯虫苯甲酰胺亚致死剂量对蜂巢小甲虫生长发育和繁殖的影响  

Effects of sublethal dosages of chlorantraniliprole on development and fecundity of Aethina tumida(Coleoptera:Nitidulidae)

作　　者：韩文素[1,3] 李健 高景林[1,3] 李翔[2] 钟义海[1,3] 王释婕 赵珊 HAN Wen-Su;LI Jian;GAO Jing-Lin;LI Xiang;ZHONG Yi-Hai;WANG Shi-Jie;ZHAO Shan(Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China;College of Animal Science and Technology,Huazhong Agricultural University,Wuhan 430070,China;Bee Industry Technology Research Center,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China)

机构地区：[1]中国热带农业科学院环境与植物保护研究所,海口571101 [2]华中农业大学动物科学技术学院,武汉430070 [3]中国热带农业科学院蜂业技术研究中心,海口571101

出　　处：《环境昆虫学报》2023年第4期1095-1102,共8页Journal of Environmental Entomology

基　　金：海南省科技专项(ZDYF2021XDNY282);国家现代农业产业技术体系资助(CARS-44-SYZ11);海南省自然科学基金(2019RC273)。

摘　　要：氯虫苯甲酰胺是我国蜂农防治蜂巢小甲虫Aethina tumida的主要杀虫剂。本研究旨在探讨氯虫苯甲酰胺对蜂巢小甲虫生长发育和繁殖的亚致死效应,为氯虫苯甲酰胺的合理使用提供一定的理论依据。本文采用饲料混毒法测定了氯虫苯甲酰胺对蜂巢小甲虫的毒力以及亚致死剂量LC_(10)和LC_(25)对蜂巢小甲虫生长发育和繁殖的影响。结果表明,氯虫苯甲酰胺对蜂巢小甲虫3龄初幼虫的LC_(50)、LC_(25)、LC_(10)分别为5.193μg/g、1.678μg/g和2.865μg/g。以氯虫苯甲酰胺亚致死剂量处理蜂巢小甲虫3龄初幼虫后,幼虫发育历期、雌预蛹期+蛹期、雄预蛹+蛹期均显著延长,且分别延长了3.38%和4.50%、10.51%和21.92%、5.26%和12.20%。LC_(10)和LC_(25)处理组幼虫的存活率均降低,尤其LC_(25)处理组与对照组存在显著差异。LC_(10)和LC_(25)处理组漫游期幼虫重、初羽化雌虫重和初羽化雄虫重相比于对照均显著降低,且分别减轻了15.45%、21.54%、17.26%和13.47%、16.47%、16.46%。LC_(10)和LC_(25)处理组的产卵前期分别为7.96 d和7.47 d,比对照组显著缩短12.36 d和12.85 d;60 d内单雌产卵总量与对照组间无显著性差异,但在产卵期5~7 d时,LC_(25)处理组的单雌产卵量显著增加。LC_(10)和LC_(25)处理组蜂巢小甲虫种群趋势指数低于对照组,但其种群趋势指数均远大于1,表明蜂巢小甲虫种群仍呈增长趋势,因此应加强蜂巢小甲虫的防控以及对氯虫苯甲酰胺的抗性监测。Chlorantraniliprole is widely used to control the small hive beetle Aethina tumida Murray by beekeepers in our country.The aim of this study is focus on the sublethal effects of chlorantraniliprole on the development and reproduction of A.tumida.The diet incorporation method was used to test the toxicity of chlorantraniliprole and to investigate the sublethal effects(LC_(10) and LC_(25))of chlorantraniliprole on development and fecundity of A.tumida.The results showed that the LC_(50),LC_(25) and LC_(10) of chlorantraniliprole on innitial 3 rd instar larvae of A.tumida were 5.193μg/g,1.678μg/g and 2.865μg/g,respectively.After treatment of 3 rd instar larvae with sublethal dosages of chlorantraniliprole,the developmental duration of larvae,prepupal+pupae period of female,and prepupal+pupae period of male was significantly prolonged by 3.38%and 4.50%,10.51%and 21.92%,5.26%and 12.20%,respectively.The survival rate of the larvae in the LC_(10) and LC_(25) treatment groups were decreased,with a significant difference observed between the LC_(25) treatment and the control group.The weight of the wandering larvae,initial emerging females and males in the LC_(10) and LC_(25) treatment groups also significantly decreased by 15.45%、21.54%、17.26%and 13.47%、16.47%、16.46%,respectively.The preoviposition period of female adults in the LC_(10) and LC_(25) treatment groups was 7.96 d and 7.47 d,which were significantly shorter by 12.36 d and 12.85 d than that of control group.It's worth noting that there were no significant differences between treated groups and the control in the total number of eggs laid per female within 60 days.However,the number of eggs laid per female in the LC_(25) group significantly increased during the 5~7 days oviposition period.The population trend index of A.tumida in the LC_(10) and LC_(25) treatment groups were lower than that in the control group,but the population trend index was substantially larger than 1.The results indicated that the population of A.tumida exposed to sublethal dos

关 键 词：蜂巢小甲虫 氯虫苯甲酰胺 亚致死效应 发育 繁殖 

分 类 号：Q965.9[生物学—昆虫学] S433[农业科学—农业昆虫与害虫防治]