不同亚型CHO宿主细胞对抗体表达的影响  

Effects of Different Sources of CHO Host Cells on Antibody Expression

作　　者：曹辉 董静 贾宇[1] 江一帆[1] CAO Hui;DONG Jing;JIA Yu;JIANG Yifan(State Key Laboratory of Antibody Drug Development,Hebei Engineering Research Center of Antibody Medicine,New Drug Research and Development Co.Ltd,North China Pharmaceutical)

机构地区：[1]华北制药集团新药研究开发有限责任公司,抗体药物河北省工程研究中心,抗体药物研究国家重点实验室,石家庄050015

出　　处：《生物技术进展》2023年第5期698-703,共6页Current Biotechnology

基　　金：河北省科技重大专项(21282401Z)

摘　　要：CHO细胞作为宿主细胞广泛应用于生物药工业化生产中。其中,CHO-K1、CHO-DG44和CHO-S是最常见的3种亚型。虽然这些亚型是从共同的原始CHO细胞分离出来的,但在不同的实验室或生物医药公司、研究人员、培养基或培养方式下连续传代、驯化和保存,使得CHO细胞积累了大量变异,导致宿主细胞应用于抗体药生产时会在细胞生长状态、抗体表达量及以糖型为代表的质量属性方面表现出较大差异。综述了CHO细胞不同亚型的染色体差异、生长状态、表达差异以及糖型差异,以期为抗体药物研发中宿主细胞的选择提供参考。CHO cells comprise a variety of lineages including CHO-K1,CHO-DG44 and CHO-S,which have been widely used in the industrial production of biological drugs.All CHO cell lines share a common ancestor,however,during the process of cell passage cultivation,cell domesticated,and preservation by different laboratories or companies,substantial genetic heterogeneity among them has been produced,that showed great differences in cell growth state,antibody titer,glycosylation and other product quality attributes.This article reviewed the difference in chromosome,growing status and expression,and glycoform in different sources of CHO host cells,which was expected to be helpful in host cell selection during antibody drug research and development process.

关 键 词：CHO细胞 抗体 表达量 糖型 

分 类 号：Q28[生物学—细胞生物学] R392-33[医药卫生—免疫学]