MTT法在中草药成分干预细胞增殖及活性中的应用  

Application of MTT Method in Intervention of Chinese Herbal Medicine Ingredients in Cell Proliferation and Activity

作　　者：任周新[1] REN Zhouxin(College of Traditional Chinese Medicine of Henan University of Chinese Medicine/Collaborative Innovation Center for the Prevention and Treatment of Respiratory Diseases with Traditional Chinese Medicine,Zhengzhou Henan China 450046)

机构地区：[1]河南中医药大学中医药科学院/呼吸疾病中医药防治省部共建协同创新中心,河南郑州450046

出　　处：《中医学报》2023年第10期2043-2049,共7页Acta Chinese Medicine

基　　金：国家自然科学基金项目(81973822);国家中医临床研究基地业务建设第二批科研专项项目(JDZX2015156)。

摘　　要：MTT实验具有快速、方便和经济性好的特点,是一种评估中草药活性成分干预细胞增殖和活性的常规方法,用于悬浮细胞和贴壁细胞,步骤略有差异。中草药分子量较大、结构复杂,某些基团与甲臜分子基团相互作用,导致最大吸收峰漂移,故检测应重视检测波长的选择。同时,应根据检测目的综合考量检测效率、成本和准确性,选择不同的对照。在结果分析中,尽量采用IC50值评估中草药成分的效应。此外,某些分子可进入细胞,其吸收峰与甲臜相同或接近,可能导致检测结果失真,清洗贴壁细胞等质控方法有时无法解决此问题,故MTT方法可分别与BrdU、3H-TdR法、直接细胞计数或台盼蓝染色法等其他评估方法联合应用,取长补短,以快速、准确、客观地反映药物干预效应。MTT experiment has the characteristics of rapidity,convenience and good economy.It is a conventional method to evaluate the intervention of Chinese herbal medicine active ingredients in cell proliferation and activity.The steps are slightly different for suspension cells and adherent cells.Chinese herbal medicine has a large molecular weight and a complex structure.Some functional groups interact with formazine molecular groups,leading to the drift of the maximum absorption peak.Therefore,attention should be paid to the selection of detection wavelength in detection.At the same time,the efficiency,cost and accuracy of the test should be comprehensively considered according to the purpose of the test,and different controls should be selected.In the analysis of results,try to use the IC50 value to evaluate the effects of Chinese herbal ingredients.In addition,some molecules can enter the cells,and their absorption peaks are the same or close to that of methazan,which may lead to distortion of the test results.Sometimes quality control methods such as cleaning adherent cells cannot solve this problem.Therefore,MTT method can be used together with other evaluation methods such as BrdU,3H TdR,direct cell counting or Trypan blue staining,to learn from each other to quickly,accurately and objectively reflect the effect of drug intervention.

关 键 词：MTT法 中草药 细胞增殖 细胞活性 

分 类 号：R285[医药卫生—中药学]