金锁固精丸加味方对链脲佐菌素诱导的糖尿病肾病大鼠的保护作用研究  

Protective Effect of Modified Jinsuo Gujing Pills on Streptozotocin-Induced Diabetic Kidney Disease in Rats

作　　者：温紫凌 梁谋[1,2] WEN Ziling;LIANG Mou(Guangzhou University of Chinese Medicine,Guangzhou Guangdong 510006,China;Shunde Hospital of Guangzhou University of Chinese Medicine,Foshan Guangdong 528300,China)

机构地区：[1]广州中医药大学,广东广州510006 [2]广州中医药大学顺德医院,广东佛山528300

出　　处：《新中医》2023年第15期1-7,共7页New Chinese Medicine

基　　金：佛山市自筹经费类科技计划项目(2020001005156)。

摘　　要：目的:观察金锁固精丸加味方对糖尿病肾病大鼠的保护作用。方法:将糖尿病肾病大鼠随机分组,分为中药常规剂量组、中药加倍剂量组、雷帕霉素组及模型对照组,并设置正常对照组。在造模给药后第12周,观察各组大鼠的一般情况,检测空腹血糖(FBG)、血尿素氮(BUN)、血肌酐(SCr)、总胆固醇(TC)、24 h尿白蛋白定量(24 hUAlb)和血清白蛋白(Alb)水平,并用过碘酸-雪夫反应(PAS)染色光镜下及电镜下观察各组大鼠肾组织病理变化。结果:生化指标方面,与雷帕霉素组比较,中药加倍剂量组的TC、SCr、BUN及24 hUAlb水平显著降低(P<0.05),中药常规剂量组的Alb、FBG、SCr、BUN及24 hUAlb水平均无明显差异(P>0.05);与中药常规剂量组比较,中药加倍剂量组的SCr、BUN、24 hUAlb明显下降(P<0.05)。病理方面,与雷帕霉素组比较,中药组可显著抑制糖尿病肾病大鼠的肾小球系膜区域增生(P<0.05),中药加倍剂量组可显著抑制肾小球肥大(P<0.05);与中药常规剂量组比较,中药加倍剂量组更能有效抑制肾小球肥大(P<0.05)。结论:金锁固精丸加味方可改善糖尿病肾病大鼠的肾功能,修复肾脏的病理损伤,且具有一定的剂量依赖。Objective:To observe the protective effect of modified Jinsuo Gujing Pills on diabetic kidney disease rats.Methods:Diabetic kidney disease rats were randomly divided into the conventional dose group of Chinese medicine,the double dose group of Chinese medicine,the Rapamycin group,and the model control group,and a normal control group was set up.After 12 weeks of modeling and administration,observe the general situation of rats in each group,detect fasting blood glucose(FBG),blood urea nitrogen(BUN),blood creatinine(SCr),total cholesterol(TC),24-hour urinary albumin(24 hUAlb),and serum albumin(Alb)levels,and observe the pathological changes of renal tissue in each group under light and electron microscopy using Periodic Acid-Schiff(PAS)staining.Results:In terms of biochemical indicators,compared with the Rapamycin group,the TC,SCr,BUN,and 24 hUAlb levels in the double dose group of Chinese medicine were significantly reduced(P<0.05),while the Alb,FBG,SCr,BUN,and 24 hUAlb levels in the conventional dose group of Chinese medicine showed no significant difference(P>0.05).Compared with the conventional dose group of Chinese medicine,the SCr,BUN,and 24 hUAlb of the double dose group of Chinese medicine decreased significantly(P<0.05).Pathologically,compared with the Rapamycin group,the Chinese medicine group could significantly inhibit the proliferation of the mesangial region of diabetic kidney disease rats(P<0.05),and the Chinese medicine double dose group could significantly inhibit the glomerular hypertrophy(P<0.05).Compared with the conventional dose group of Chinese medicine,the double dose group of Chinese medicine was more effective in inhibiting glomerular hypertrophy(P<0.05).Conclusion:The modified Jinsuo Gujing Pills can improve renal function and repair pathological damage in diabetic kidney disease rats,and has a certain dose-dependent effect.

关 键 词：糖尿病肾病 金锁固精丸加味方 雷帕霉素 中医药 肾功能 

分 类 号：R587.2[医药卫生—内分泌]