蜂毒肽对前列腺炎大鼠模型的抗炎和抗增生作用的研究  

Anti-inflammatory and anti-proliferative effects of melittin on rat model of prostatitis

作　　者：周成刚 ZHOU Cheng-gang(Second Department of Urology Surgery,The First Affiliated Hospital of Kunming Medical University,Kunming 650031,Yunnan Province,China)

机构地区：[1]昆明医科大学第一附属医院泌尿外二科,云南昆明650031

出　　处：《中国临床药理学杂志》2023年第15期2193-2197,共5页The Chinese Journal of Clinical Pharmacology

基　　金：云南省卫生科技计划基金资助项目(2017NS151)。

摘　　要：目的探讨蜂毒肽对前列腺炎的治疗作用,及其相关的作用机制。方法雄性SD大鼠摘除睾丸后,皮下注射雌二醇诱导前列腺炎模型。造模成功后,随机分为模型组和低、中、高剂量实验组,每组10只;另取10只正常大鼠作为空白组。低、中、高剂量实验组分别给予前列腺腹叶注射0.25、0.50和1.00 mg·kg^(-1)的蜂毒肽0.2 mL。空白组和模型组均给予前列腺腹叶注射0.9%NaCl 0.2 mL。5组大鼠均治疗4周。用酶联免疫吸附试验法检测血清中白细胞介素(IL)-6和IL-8水平,用实时荧光定量聚合酶链反应法检测单核细胞趋化蛋白-1(MCP-1)和血管细胞黏附分子-1(VCAM-1)mRNA的表达水平,用蛋白质印迹法检测极迟抗原-4(VLA-4)和成纤维细胞生长因子受体-1(FGFR-1)蛋白的表达水平,用Masson三色染色观察前列腺的纤维化程度。结果中、高剂量实验组和模型组、空白组的血清IL-6水平分别为(41.79±2.12)、(32.65±2.35)、(59.15±3.79)和(21.75±1.38)pg·mL^(-1),IL-8水平分别为(130.86±5.86)、(119.24±9.06)、(217.45±5.86)和(96.42±6.49)pg·mL^(-1),MCP-1 mRNA相对表达量分别为1.81±0.10、1.42±0.12、4.45±0.28和1.00±0.04,VCAM-1 mRNA相对表达量分别为1.76±0.12、1.22±0.08、3.32±0.30和1.00±0.03,VLA-4蛋白相对表达水平分别为0.52±0.05、0.44±0.03、0.85±0.07和0.34±0.04,FGFR-1蛋白相对表达水平分别为0.69±0.05、0.57±0.03、0.97±0.06和0.44±0.04,纤维化面积分别为(10.33±1.25)%、(5.33±0.28)%、(22.80±1.58)%和(1.92±0.10)%。中、高剂量实验组的上述指标与模型组比较,差异均有统计学意义(均P<0.05)。结论蜂毒肽对前列腺炎大鼠具有较好的抗炎和抗增生作用,其可通过调节前列腺组织中趋化因子、黏附分子和成纤维细胞生长因子的水平来发挥治疗作用。Objective To explore the therapeutic effect of melittin on prostatitis and its related mechanism.Methods Prostatitis model was induced by subcutaneous injection of estradiol after testicular removal in male SD rats.After successful modeling,the rats were randomly divided into model group and experimental-L,-M,-H groups with 10 rats in each group,and another 10 normal rats were selected as blank group.The experimental-L,-M,-H groups were injected with 0.25,0.50 and 1.00 mg·kg^(-1) melittin 0.2 mL into the ventral lobe of the prostate,respectively.0.9%NaCl 0.2 mL was injected into ventral lobe of prostate in both blank group and model group.All the rats in 5 groups were treated for 4 weeks.The levels of serum interleukin(IL)-6 and IL-8 were detected by enzyme-linked immunosorbent assay.The mRNA expression levels of monocyte chemotactic protein-1(MCP-1)and vascular cell adhesion molecule-1(VCAM-1)were detected by real-time fluorescence quantitative polymerase chain reaction.The expression levels of very late antigen-4(VLA-4)and fibroblast growth factor receptor-1(FGFR-1)were detected by Western blot.The degree of prostate fibrosis was observed by Masson trichrome staining.Results The levels of serum IL-6 in the experimental-M,-H groups,model group and blank group were(41.79±2.12),(32.65±2.35),(59.15±3.79)and(21.75±1.38)pg·mL^(-1);the IL-8 levels were(130.86±5.86),(119.24±9.06),(217.45±5.86)and(96.42±6.49)pg·mL^(-1);the relative expression levels of MCP-1 mRNA were 1.81±0.10,1.42±0.12,4.45±0.28 and 1.00±0.04;the relative expression levels of VCAM-1 mRNA were 1.76±0.12,1.22±0.08,3.32±0.30 and 1.00±0.03;the relative expression levels of VLA-4 protein were 0.52±0.05,0.44±0.03,0.85±0.07 and 0.34±0.04;the relative expression levels of FGFR-1 protein were 0.69±0.05,0.57±0.03,0.97±0.06 and 0.44±0.04;the fibrosis areas were(10.33±1.25)%,(5.33±0.28)%,(22.80±1.58)%and(1.92±0.10)%,respectively.Compared with model group,the above indexes in the experimental-M,-H groups had statistically significant

关 键 词：蜂毒肽 前列腺炎 抗炎 抗增生 趋化因子 黏附分子 成纤维细胞生长因子 

分 类 号：R28[医药卫生—中药学]