耐盐好氧反硝化菌Vibrio alginolyticus YT-3的固定化及对海水养殖废水的脱氮处理  

Immobilization of a Halotolerant Aerobic Denitrifier Vibrio Alginolyticus YT-3 and its Application in Denitrification of Mariculture Wastewater

作　　者：付雨潼 王帅杰[1,2] 叶菲 王露 张颖超 齐海云 FU Yutong;WANG Shuaijie;YE Fei;WANG Lu;ZHANG Yingchao;QI Haiyun(School of Environmental and Chemical Engineering,Yanshan University,Hebei Key Laboratory of Heavy Metal Deep-Remediation in Water and Resource Reuse,Qinhuangdao,China,066004;Hebei University of Environmental Engineering,Hebei Key Laboratory of Agroecological Safety,Qinhuangdao,China,066102)

机构地区：[1]燕山大学环境与化学工程学院河北省水体重金属深度修复与资源利用重点实验室,河北秦皇岛066004 [2]河北环境工程学院河北省农业生态安全重点实验室,河北秦皇岛066102

出　　处：《水处理技术》2023年第9期64-70,共7页Technology of Water Treatment

基　　金：河北省自然科学基金(B2020203024);河北省农业高质量发展关键共性技术攻关专项(21327312D);河北省重点研发计划项目(22327309D)。

摘　　要：以驯化分离得到的一株耐盐好氧反硝化细菌Vibrio alginolyticus YT-3为研究对象,系统研究了其在不同C/N、盐度、温度以及pH下的脱氮性能。同时,以陶粒为载体,对Vibrio alginolyticus YT-3进行固定化,优化固定化条件,并初步确定固定化Vibrio alginolyticus YT-3对实际海水养殖废水的脱氮效果。结果表明:Vibrio alginolyticus YT-3在C/N=15~20、25~30℃、中性和偏碱性条件及较宽的盐度范围内(0~40‰)具备较强的脱氮能力。最优的固定化条件为陶粒粒径2~4 mm、固菌比为1:3(质量:体积,g:mL)、固定化时间24 h。与游离态相比,固定化后的Vibrio alginolyticus YT-3具有更宽的温度和pH适宜范围。经固定化Vibrio alginolyticus YT-3的脱氮处理,海水养殖废水中无机氮浓度下降至0.32 mg/L,总氮浓度下降至1.95 mg/L,氨氮、硝态氮、总氮的去除率达100%、85.03%、92.52%。With Vibrio alginolyticus YT-3 as the research object,a halotolerant aerobic denitrifier domesticated and isolated,its denitrification performance under different environmental conditions of C/N,salinity,temperature and initial pH were systematically studied.Meanwhile,Vibrio alginolyticus YT-3 was immobilized with the ceramsite as the carrier.The immobilization conditions were optimized,and the denitrification effect of the immobilized Vibrio alginolyticus YT-3 on the actual mariculture wastewater was preliminarily determined.The results showed that Vibrio alginolyticus YT-3 has efficient nitrogen removal ability under the conditions of C/N=15~20,25~30℃,neutral and weakly alkaline conditions,and a wide range of salinity(0~40‰).The optimal immobilization conditions are as follows:the ceramsite particle size is 2~4 mm,the solid-bacteria ratio is 1:3(mass:volume,g/mL),and the immobilization time is 24 h.Compared with the free Vibrio alginolyticus YT-3,the immobilized Vibrio alginolyticus YT-3 has a wider suitable range of temperature and pH.After the nitrogen removal treatment by the immobilized Vibrio alginolyticus YT-3,the concentrations of inorganic nitrogen and total nitrogen in the mariculture wastewater decreased to 0.32 mg/L and 1.95 mg/L respectively,and the removal ratios of NH4+-N,NO3--N and TN reached 100%,85.03%,and 92.52%.

关 键 词：海水养殖废水 好氧反硝化菌 耐盐 固定化 

分 类 号：X55[环境科学与工程—环境工程]