痰火方调节M1型小胶质细胞活化及减轻脑缺血大鼠脑组织损伤作用的研究  

Study on the mechanism of Tanhuo Prescription in the regulation of M1-type microglia activation and reducing the damage of brain tissue in rats with cerebral ischemia

作　　者：赵婷[1] 李慢中 冯雪枫 陆允 李明聪 高利[2] 赵晖[1] Zhao Ting;Li Manzhong;Feng Xuefeng;Lu Yun;Li Mingcong;GaoLi;Zhao Hui(School of Traditional Chinese Medicine,Capital Medical University,Beijing 100069,China;Department of Neurology,Xuanwu Hospital,Capital Medical University,Beijing 100053,China)

机构地区：[1]首都医科大学中医药学院,北京100069 [2]首都医科大学宣武医院神经内科,北京100053

出　　处：《国际中医中药杂志》2023年第9期1105-1112,共8页International Journal of Traditional Chinese Medicine

基　　金：北京市科学技术委员会“十病十药”研发专项(Z171100001717012)。

摘　　要：目的观察痰火方调节M1型小胶质细胞活化及减轻脑缺血大鼠脑组织损伤的作用及机制。方法将雄性SD大鼠按随机数字表法分为假手术组、模型组、痰火方高剂量组(3.68 g/kg)、痰火方中剂量组(1.84 g/kg)、痰火方低剂量组(0.92 g/kg)及金纳多组(0.06 g/kg)。除假手术组外,其余各组采用大脑中动脉线栓法建立脑缺血大鼠模型。采用平衡木行走实验评价大鼠运动平衡能力,磁共振成像(MRI)T2 mapping检测脑缺血大鼠脑组织损伤,快蓝染色(LFB)检测神经纤维损伤,HE染色检测神经细胞损伤情况,采用免疫荧光技术检测小胶质细胞标志物离子钙结合衔接分子1(Iba-1)及CD16/Iba-1阳性细胞数。结果与模型组比较,痰火方高剂量组、金纳多组大鼠缺血24、48、72 h平衡木行走能力评分提高(P<0.05或P<0.01),痰火方低、中剂量组大鼠缺血72 h平衡木行走能力评分提高(P<0.01);痰火方高剂量组、金纳多组大鼠梗死灶周围皮层、纹状体脑区T2值降低(P<0.05或P<0.01),痰火方低、中剂量组大鼠梗死灶周围纹状体T2值降低(P<0.05);痰火方高、低剂量组及金纳多组大鼠梗死灶周围皮层、纹状体及外囊LFB积分光密度增加(P<0.01),痰火方中剂量组大鼠梗死灶周围纹状体的LFB积分光密度增加(P<0.01);痰火方低、中、高剂量组大鼠梗死灶周围纹状体病理损伤程度降低,细胞密度增加(P<0.05或P<0.01),金纳多组大鼠梗死灶周围皮层、纹状体细胞密度增加(P<0.01或P<0.05);痰火方高、中剂量组及金纳多组大鼠梗死灶周围皮层、纹状体Iba-1及CD16/Iba-1阳性细胞数减少(P<0.01),痰火方低剂量组大鼠梗死灶周围皮层、纹状体CD16/Iba-1阳性细胞数减少(P<0.01),痰火方低剂量组大鼠梗死灶周围纹状体Iba-1阳性细胞数减少(P<0.01)。结论痰火方可改善脑缺血大鼠神经功能缺损症状,减轻缺血梗死灶周围脑区神经病理学损伤,抑制M1型小胶质细胞活化。Objective To observe the effect and mechanism of Tanhuo Prescription on regulating the activation of M1 microglia and alleviating brain tissue injury in rats with cerebral ischemia.Methods Male SD rats were divided into sham-operation group,model group,Tanhuo Prescription high-(3.68 g/kg),medium-(1.84 g/kg),low-dosage(0.92 g/kg)groups,and ginaton group(0.06 g/kg)using random number table method.Except for the sham-operation group,the other groups established cerebral ischemia rat models using the middle cerebral artery occlusion method.The balance beam walking test was used to evaluate the symptoms of neurological deficit.MRI-T2 mapping was used to measure the damage to brain tissue.LFB staining was used to observe the damage to nerve fibers.HE staining was used to observe the damage to nerve cell,and Iba-1 and CD16/Iba-1 immunofluorescence staining were used to observe the condition of microglial activation.Results Compared with the model group,the scores of balance beam walking ability of rats in Tanhuo Prescription high-dose group and ginaton group at 24 h,48 h and 72 h after ischemia were significantly improved(P<0.05,P<0.01).The scores of balance beam walking ability of rats in Tanhuo Prescription low-and medium-dose groups at 72 h after ischemia were improved(P<0.01).Compared with the model group,the T2 values of the cortex and striatum around the infarct of rats in Tanhuo Prescription high-dose group and ginaton group were significantly reduced(P<0.05,P<0.01),and the T2 values of the striatum around the infarct of rats in Tanhuo Prescription low-and medium-dose groups were significantly reduced(P<0.05).Compared with the model group,the LFB IOD of the cortex,striatum and outer capsule around the infarct decreased in the Tanhuo Prescription high-,low-dose group and ginaton group(P<0.01).The LFB IOD of striatum around infarct decreased in medium-dose Tanhuo Prescription group(P<0.01).Compared with the model group,the pathological injury degree of the striatum around the infarct of rats in Tanhuo Prescription

关 键 词：脑缺血 痰火方 小神经胶质细胞 磁共振成像 大鼠 

分 类 号：R285.5[医药卫生—中药学]