不同部位平车前、车前及市售混用药材鉴别与质量评价  

Identification and quality evaluation of mixed herb in different parts of Plantago depressa Willd.and Plantago asiatica L.

作　　者：洪婉敏 姚晓璇 程钰洁 莫秋怡 徐杰 汪凯东 梁志毅 Hong Wanmin;Yao Xiaoxuan;Cheng Yujie;Mo Qiuyi;Xu Jie;Wang Kaidong;Liang Zhiyi(Guangdong Yifang Pharmaceutical Co.Ltd.,Key Lab of TCM Formulation Granules Enterprises in Guangdong Province,Foshan 528244,China)

机构地区：[1]广东一方制药有限公司,广东省中药配方颗粒企业重点实验室,佛山528244

出　　处：《国际中医中药杂志》2023年第9期1134-1143,共10页International Journal of Traditional Chinese Medicine

基　　金：国家工业和信息化部2019年产业技术基础公平服务平台项目(2019-00902-1-2);佛山市科技创新项目(2120001002309)。

摘　　要：目的建立车前草药材的指纹图谱。方法采用超高效液相色谱法测定指纹图谱,对不同药用部位、不同来源药材的指纹图谱峰面积进行方差分析及独立样本t检验,采用SIMCA14.1进行主成分分析、聚类分析、偏最小二乘回归分析(PLS-DA)等化学模式识别分析,采用CRITIC法计算指标权重并结合灰色关联度综合评价车前草药材的质量。结果平车前全草、茎、叶、穗的指纹图谱各标定了24、16、23、22个共有峰,车前全草、根茎、叶、穗的指纹图谱各标定了22、10、16、22个共有峰,市售混用车前草药材指纹图谱共标定了23个共有峰。共指认10个色谱峰。方差分析结果显示,平车前与车前不同部位色谱峰峰面积存在差异。结合PLS-DA可知,平车前、车前与市售混用车前草样品分类中有16个重要的特征性指标,重要程度排名依次为峰3、峰8、峰28、峰12、峰14、峰7、峰5、峰17、峰6、峰19、峰23、峰11、峰22、峰27、峰9、峰16。CRITIC法结合灰色关联度的综合质量评价结果显示,21批平车前、车前及市售混用车前草药材中,车前药材的整体质量最优。结论市面上的车前草药材存在基原混用的情况,本研究建立的指纹图谱可用于不同来源车前草的鉴别及质量评价。Objective To establish the fingerprints of Plantaginis Herba.Methods The fingerprints were determined by UPLC.The peak areas of fingerprints of different parts and origins were analyzed by variance analysis and independent sample t-test.PCA,HCA,PLS-DA and other chemical patterns were analyzed by Simca14.1.The index weight was calculated by CRITIC,and the quality of plantain evaluation was combined with grey correlation degree.Results The fingerprints of grass,stem,leaf and spike of Plantago depressa Willd.calibrated for 24,16,23 and 22 common peaks.The fingerprints of grass,stem,leaf and spike of Plantaginis Herba calibrated for 22,10,16 and 22 common peaks,and the fingerprints of commercial mixed plantain calibrated for 23 common peaks.10 peaks were identified.The analysis of variance showed that there were differences in chromatographic peak areas between different parts of Plantago asiatica L.and Plantago depressa Willd..And combinedede with PLS-DA,it showed that there were 16 important characteristic indexes in the classification,and the importance ranking was peak 3,8,28,12,14,7,5,17,6,19,23,11,22,27,9,16.The quality evaluation results of critical method combined with grey correlation degree showed that among Plantago depressa Willd.,Plantago asiatica L.and commercial mixed plantain herbs,the quality of Plantago asiatica L.was the best.Conclusion The mixture of plantain exists in the market.The fingerprints established in this study can be used for the identification and quality evaluation of Plantaginis Herba from different sources.

关 键 词：车前草 指纹图谱 中药鉴定 CRITIC法 灰色关联度 

分 类 号：R282.5[医药卫生—中药学]