Unveiling the interaction mechanisms of key functional microorganisms in the partial denitrification-anammox process induced by COD  

作　　者：Guangjiao Chen Lan Lin Ying Wang Zikun Zhang Wenzhi Cao Yanlong Zhang 

机构地区：[1]Key Laboratory of Marine Environmental Science,College of the Environment and Ecology,Xiamen University,Xiamen 361102,China [2]Fujian Key Laboratory of Coastal Pollution Prevention and Control,College of Environment and Ecology,Xiamen University,Xiamen 361102,China [3]Department of Civil and Environmental Engineering,Graduate School of Engineering,Tohoku University,Sendai,Miyagi 980-8579,Japan

出　　处：《Frontiers of Environmental Science & Engineering》2023年第8期153-165,共13页环境科学与工程前沿（英文）

基　　金：the National Natural Science Foundation of China(No.51808473);the National Key R&D Program of China(No.2017YFE0127300);the Science Foundation of Fujian Province(No.2020J01046);the Fundamental Research Funds for the Central Universities(No.20720200112).

摘　　要：Partial denitrification-anammox(PD-anammox)is an innovative process to remove nitrate(NO_(3)^(–)–N)and ammonia(NH4+–N)simultaneously from wastewater.Stable operation of the PD-anammox process relies on the synergy and competition between anammox bacteria and denitrifiers.However,the mechanism of metabolic between the functional bacteria in the PD-anammox system remains unclear,especially in the treatment of high-strength wastewater.The kinetics of nitrite(NO_(2)^–N)accumulation during denitrification was investigated using the Michaelis-Menten equation,and it was found that low concentrations of NO_(3)^–N had a more significant effect on the accumulation of NO_(2)^–N during denitrification.Organic matter was a key factor to regulate the synergy of anammox and denitrification,and altered the nitrogen removal pathways.The competition for NO_(2)^–N caused by high COD concentration was a crucial factor that affecting the system stability.Illumina sequencing techniques demonstrated that excess organic matter promoted the relative abundance of the Denitratesoma genus and the nitrite reductase gene nirS,causing the denitrifying bacteria Denitratisoma to compete with Cadidatus Kuenenia for NO_(2)^–N,thereby affecting the stability of the system.Synergistic carbon and nitrogen removal between partial denitrifiers and anammox bacteria can be effectively achieved by controlling the COD and COD/NO_(3)^(–)N.

关 键 词：PD-anammox process Nitrite accumulation COD Microbial interaction 

分 类 号：X703[环境科学与工程—环境工程]