稀土纳米探针用于同波长比率测温及生物成像  

Rare Earth Nanoprobes for the Same-wavelength Ratiometric Temperature Measurement and Biological Imaging

作　　者：武玉凯 朱幸俊 WU Yu-kai;ZHU Xing-jun(School of Physical Science and Technology,ShanghaiTech University,Shanghai 201210,China)

机构地区：[1]上海科技大学物质科学与技术学院,上海201210

出　　处：《稀土》2023年第4期235-246,I0008,共13页Chinese Rare Earths

基　　金：国家自然科学基金青年项目(82001945)。

摘　　要：生物体温度检测在疾病的诊断和治疗以及生物学机制研究中具有重要的意义。体内温度检测的现有方法和技术仍然存在着检测不直观、组织深度小或者侵入性较高的问题。发光纳米温度探针是一种新兴的测温技术,近红外比率型发光温度探针具备测温速度快,可视化以及空间精度高的优势。然而由于检测波长的差异,测温的准确性会受到组织吸收散射的干扰。本研究通过稀土发光纳米复合材料,构建了一种镱、铒离子共掺杂的双激发同波长发射的稀土发光纳米温度探针。以铒离子作为发光中心,在808 nm和980 nm激发下产生同一种位于近红外第二窗口的1550 nm发射,作为比率测温探针的工作波长和参比波长,避免了波长差异造成的生物组织消光系数的误差,有望实现更高测温准确度。研究结果表明,所设计的双激发同波长稀土发光纳米探针具有均一的粒径和形貌,较强的发射强度以及良好生物相容性,1550 nm的发射强度比率能够在10℃~90℃范围实现温度检测。进一步的活体成像结果验证了纳米探针的高空间分辨的成像效果。本研究将为生物体内温度检测提供一种新的思路。Organism temperature detection is of great significance in the diagnosis and treatment of diseases and the study of biological mechanisms.The prior methods and technologies for temperature detection in the living body still have the prob-lems of unintuitive detection,small tissue depth or high invasiveness.Luminescent nanothermometers are an emerging technol-ogy for temperature measurement.Near-infrared ratiometric luminescent temperature probes have the advantages of fast temper-ature measurement,visualization and high spatial precision.However,due to the difference in detection wavelengths,the ac-curacy of temperature measurement will be interfered by tissue absorption and scattering.In this study,a rare earth lumines-cent nanothermometer with dual-excitation and the same-wavelength emission was constructed by using Yb^(3+)and Er^(3+)co-doped rare earth nanocomposites.With erbium ions as the luminescent center,the same 1550 nm emission located in the second near-infrared window is generated under excitation at 808 nm and 980 nm,which is used as the working wavelength and refer-ence wavelength of the ratiometric temperature measurement probe,avoiding the extinction of biological tissues caused by wavelength differences.The error of the coefficient is expected to achieve higher temperature measurement accuracy.The re-search results show that the designed rare earth luminescent nanoprobe with dual-excitation and emission at the same wavelength has uniform particle size and shape,strong emission intensity and good biocompatibility,and the ratio of emission intensity at 1550 nm can be adjusted at 10℃to 90℃for temperature detection.Further in vivo imaging results verified the high spatial res-olution imaging effect of nanoprobes.This study will provide a new idea for temperature detection in living organisms.

关 键 词：稀土发光 近红外 发光比率 温度检测 

分 类 号：TD955[矿业工程—选矿]