基于拉曼光谱技术的精子评筛研究进展  

Recent Progress in Sperm Evaluation and Screening Based on Raman Spectroscopy

作　　者：黄祖芳 李玉玲 杜生荣[2] 孙艳[2] 王佳睿 张群 陈荣 Huang Zufang;Li Yuling;Du Shengrong;Sun Yan;Wang Jiarui;Zhang Qun;Chen Rong(College of Photonic and Electronic Engineering,Key Laboratory of Opto-Electronic Science and Technology for Medicine of Ministry of Education,Fujian Provincial Key Laboratory for Photonics Technology,Fujian Normal University,Fuzhou 350117,Fujian,China;Reproductive Center,Fujian Maternity and Child Health Hospital,Fuzhou 350001,Fujian,China)

机构地区：[1]福建师范大学光电与信息工程学院,医学光电科学与技术教育部重点实验室,福建省光子技术重点实验室,福建福州350117 [2]福建省妇幼保健院辅助生殖中心,福建福州350001

出　　处：《中国激光》2023年第15期90-100,共11页Chinese Journal of Lasers

基　　金：国家自然科学基金(62275049,11874006,82170908);福建省自然科学基金重点项目(2022J02024)。

摘　　要：全球成年男性的精子质量呈明显下降趋势。精子质量下降与男性不育虽并非线性关系,但显然与低生育力密切相关。辅助生殖技术针对不育男性精子水平低、形态和运动活力异常等常见问题,通过评估和筛选优质精子,提供了解决男性不育问题的主要技术方案。现有临床研究局限于精子形貌和活力等特征参数的筛选,缺乏针对精子DNA损伤情况的无损评筛手段。本文首先介绍了精子无损评筛技术在辅助生殖领域男科的应用需求以及显微拉曼光谱技术的基本原理,继而对基于拉曼光谱技术的精子研究进行了综述,综合分析探讨了精子的拉曼光谱检测和光谱特征,聚焦精子DNA损伤的拉曼光谱响应问题,最后讨论和展望了精子无损评筛技术的发展前景。Significance The quality of sperm in fertile men worldwide is deteriorating.Although sperm quality is not directly related to male infertility,it is strongly linked to subfertility.Assisted reproductive technology addresses common male infertility issues,such as low sperm count,abnormal morphology,and motility,and it is the primary technological solution to male infertility through the evaluation and screening of high-quality sperm.However,current clinical research is limited to screening sperm morphology and motility,and there is a lack of non-destructive screening methods for evaluating sperm DNA damage.This review article first introduces the demand for non-destructive sperm screening technology in assisted reproduction and the basic principle of micro-Raman spectroscopy,and then summarizes current Raman-based research progress on sperm.Next,a comprehensive analysis and discussion of Raman detection and Raman spectral characteristics of sperm cells are conducted,emphasizing the Raman response to sperm DNA damage.Finally,the potential for applied research in non-destructive sperm evaluation and screening is discussed and explored.Progress Raman-based sperm analysis is still in the initial stage of development,but the unique advantages of Raman spectroscopy have opened up new routes for sperm characterization.In 2009,Huser et al.used micro-Raman spectroscopy to correlate the spectral response of chromatin differences with sperm morphology.Meister et al.achieved Raman imaging characterization of sperm nucleus,neck,and mitochondria in sperm cells.By simulating the damaging effect of UV radiation on different sperm organelles,it was verified that micro-Raman spectroscopy is expected to provide a new method for rapid assessment of sperm mitochondrial status.Subsequently,Mallidis et al.reported that the damage to sperm DNA due to UV radiation is mainly reflected in the intensity change of the PO4 skeleton peak(1042 cm-1)attributed to DNA.Additionally,the spectral response of sperm DNA damage due to UV light was only

关 键 词：医用光学 拉曼光谱 精子 DNA损伤 评估筛选 统计分析 

分 类 号：Q492[生物学—生理学]