曲线型微纳拓扑结构的制备及细胞行为调控  

Fabrication of Curved Micro-Nano Topography Structures and Regulation of Cell Behavior

作　　者：郭敏 刘享洋 董贤子[1] 刘洁[1] 金峰[1] 郑美玲[1] Guo Min;Liu Xiangyang;Dong Xianzi;Liu Jie;Jin Feng;Zheng Meiling(Key Laboratory of Bionic Materials and Interface Science,Technical Institute of Physics and Chemistry,Chinese Academy of Sciences,Beijing 100190,China;School of Future Technology,University of Chinese Academy of Sciences,Beijing 101407,China)

机构地区：[1]中国科学院理化技术研究所仿生材料与界面科学重点实验室,北京100190 [2]中国科学院大学未来技术学院,北京101407

出　　处：《中国激光》2023年第15期191-201,共11页Chinese Journal of Lasers

基　　金：国家自然科学基金(61975213,61475164);中国科学院国际伙伴计划(GJHZ2021130);中奥国际合作项目(GJHZ1720);国家重点研发计划(2016YFA0200501)。

摘　　要：生物材料的表面拓扑结构能够显著影响细胞的黏附、增殖、迁移和分化等行为.为有效模拟体内细胞微环境,利用飞秒激光无掩模光学投影光刻技术制备了一系列曲线型拓扑结构.结果表明:细胞在沟槽、折线和三种不同曲率的波浪形拓扑结构上严格按照拓扑结构形貌进行生长、迁移.当波浪形结构曲率过大时,细胞改变原有的迁移方向,产生沿弯曲方向的迁移行为.共聚焦荧光显微图像显示:细胞在折线结构和波浪线结构的拐角区域发生骨架重排,相较于线区域细胞圆度增加.据此提出了细胞在曲线型拓扑结构上的迁移机制.该研究揭示了细胞对曲线型拓扑结构的响应机制,将为体外植入材料的设计提供科学依据.Objective The fields of tissue engineering and regenerative medicine have seen significant advancements,prompting extensive research into interactions between biomaterials and cells.Factors,such as surface chemical composition,stiffness,and topography,can influence cell adhesion,proliferation,migration,and differentiation.Advanced micro-nanofabrication technologies have made it possible to study cell-responsive topographical structures.Many cell types exhibit contact guidance behavior on groove structures,which in turn affects cell function.However,in vivo,normal tissues often exhibit curved and wavy extracellular matrix(ECM)structures,which differ significantly from previously fabricated regular topographies.Hence,in this study,maskless optical projection lithography(MOPL)was utilized to fabricate broken lines and wavy structures with varying curvatures.Groove structures were also created as controls.We examined cell migration behavior and morphological changes on the fabricated structures,proposing a cell migration mechanism specific to curved structures.This research aims to enhance our understanding of cell-surface topography interactions and provide valuable insights for designing implant materials.Methods A series of micro-nano topography structures were successfully created using maskless optical projection lithography.The surface morphologies of these structures were characterized via scanning electron microscopy(SEM)and atomic force microscopy(AFM).To promote cell adhesion,the fabricated structures were treated with O2 plasma and coated with poly-D-lysine(PDL).Cells were then cultured on the topography structures,and their migration behavior was monitored using a live cell station.After cell culture on the topography structures for 24 h,the cells were fixed and labeled with DAPI and phalloidin fluorescent probes for nuclei and actin,respectively.Quantitative analysis of cell roundness,cell perimeter,and cell spreading area was conducted to determine changes in cell morphology.The fabricated topography st

关 键 词：医用光学 飞秒激光 无掩模光学投影光刻 曲线型拓扑结构 细胞迁移 细胞骨架 

分 类 号：O436[机械工程—光学工程]