转基因大豆生信分析平台的构建及口岸初步应用  

The Construction and Preliminary Application of Transgenic Soybean Bioinformatics Analysis Platform at Ports

作　　者：杜鹃 刘誉[1] 刘振宇[1] 马鑫淼 李浩辰 马毅 董志珍 DU Juan;LIU Yu;LIU Zhen-Yu;MA Xin-Miao;LI Hao-Chen;MA Yi;DONG Zhi-Zhen(Tianjin International Travel Healthcare Center(Tianjin Customs Port Outpatient Departments),Tianjin 300456)

机构地区：[1]天津国际旅行卫生保健中心(天津海关口岸门诊部),天津300456

出　　处：《中国口岸科学技术》2023年第7期72-78,共7页China Port Science and Technology

基　　金：海关总署科研项目(2021HK262)。

摘　　要：本研究立足海关业务特点和实际需求,运用计算存储服务器整合和数据挖掘应用等技术,研究转基因大豆数据库及数据分析方法,建立基于可视化操作的转基因大豆生物信息分析平台。基于长期收集和积累的转基因植物基因组数据库,构建大豆泛基因组参考基因,结合大豆各品系标准样本,通过对大豆样品进行测序和序列分析,将其中的可疑序列进行筛选、组装后,再根据统计阈值参数判断待鉴定样本是否为转基因品种/品系,实现通过基因测序数据对转基因大豆进行高效鉴别。依托分布式云计算技术建立转基因大豆分析平台,形成基因测序数据分析方法,提高转基因大豆鉴别速度和精度。构建了具有可视化、操作简单、功能强大、数据安全性和信息保密性强等优点的转基因大豆生信分析平台,该分析平台简化了转基因大豆数据分析操作,可视化编排的流程为检测技术人员进行数据分析操作提供了便利,一键操作即可实现转基因大豆数据分析,快速、精准鉴别转基因大豆非法序列。Besed on the characteristics and practical needs of customs operations,this research uses technologies such as computational storage server integration and data mining applications,studies genetically modiffed(GM)soybean databases and data analysis methods,and establishes a visually-operated GM soybean bioinformatics analysis platform.Based on the long-term collection and accumulation of the genetically modified plant genome database,a pan-genome reference gene of soybean is constructed.Combining the standard samples of various soybean strains,the sequencing and sequence analysis of soybean samples are performed After screening and assembling the suspicious sequences,the statistical threshold parameters are used to determine whether the samples are genetically modiffed varieties/strains,thereby achieving efficient identiffcation of GM soybeans through gene sequencing data.Relying on distributed cloud computing technology to build a GM soybean analysis platform,it forms a gene sequencing data analysis method,and improves the speed and accuracy of GM soybean identiffcation.A GM soybean bioinformatics analysis platform with advantages of visualization,simple operation,powerful functions,strong data security and information conffdentiality has been built.This analysis platform simpliffes the operation of GM soybean data analysis,with visually arranged process facilitating the data analysis operation of detection technicians.With only one-click,GM soybean data analysis can be realized,and illegal sequences of GM soybeans can be quickly and accurately identiffed.

关 键 词：转基因大豆 分析平台 生信分析 基因组数据库 

分 类 号：S41[农业科学—植物保护]