异养氨氧化细菌对多花黑麦草促生的影响  

Effect of Heterotrophic AOB on the Growth-promoting of Lolium multiflorum

作　　者：李亚楠 王海红 吴迪 李理想 朱喜霞 亓琳[1] 张明明[1] 王晓凌[1] LI Ya’nan;WANG Haihong;WU Di;LI Lixiang;ZHU Xixia;QI Lin;ZHANG Mingming;WANG Xiaoling(College of Agriculture/College of Tree Peony,Henan University of Science and Technology,Luoyang,Henan 471003;College of Life Sciences,Yulin University,Yulin,Shaanxi 719000;Yindu District Bureau of Agriculture and Rural Affairs of Anyang City,Anyang,Henan 455000)

机构地区：[1]河南科技大学农学院,河南洛阳471003 [2]榆林学院生命科学学院,陕西榆林719000 [3]安阳市殷都区农业农村局,河南安阳455000

出　　处：《中国农学通报》2023年第27期157-164,共8页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金“根叶互作诱导的细胞分裂素对多花黑麦草持续性再生的调控机制”(U1304326);河南省科技创新杰出青年基金“根源细胞分裂素对旱后复水玉米补偿性生长的调控机制”(174100510004)。

摘　　要：旨在揭示土壤氨氧化细菌(Ammonia oxdizing bacteria,AOB)菌株对多花黑麦草(Lolium multiflorum Lam.)的促生效果,以盆栽多花黑麦草为试验材料,共设置4个处理:未添加硝化抑制剂和未接种、接种AOB菌株后再生、添加硝化抑制剂后再生、添加硝化抑制剂后接种AOB菌株再生。结果表明,与未添加抑制剂和未接种再生相比,接种AOB菌株的多花黑麦草再生叶生物量增加23.53%~48.63%,叶片净光合率增加27.91%~48.54%,根际土壤硝化速率增加43.01%~144.21%;土壤中添加硝化抑制剂抑制了多花黑麦草的促生,而接种S2_8_1菌株后,多花黑麦草促生效果均优于添加硝化抑制剂处理。综上,接种S2_8_1后促进了多花黑麦草根际硝化作用,刺激NO_(3)^(-)含量的增加,光合作用增强,进而促进多花黑麦草的促生效果。This study aimed to elucidate the growth-promoting effects of Ammonia oxidizing bacteria(AOB)strains on Italian ryegrass(Lolium multiflorum Lam.).Pot-grown Italian ryegrass was used as the experimental material,four treatments were established:no nitrification inhibitor and no inoculation,inoculation with AOB strains after regeneration,regeneration after adding a nitrification inhibitor,and inoculation with AOB strains after adding a nitrification inhibitor.The results showed that,compared to the non-inhibited and noninoculated regeneration,Italian ryegrass inoculated with AOB strains exhibited an increase of 23.53%to 48.63%in leaf biomass,27.91%to 48.54%in net photosynthetic rate,and 43.01%to 144.21%in rhizosphere soil nitrification rate.The addition of a nitrification inhibitor in the soil suppressed the growth promotion of Italian ryegrass.However,after inoculating with the S2_8_1 strain,the growth-promoting effects were superior to those of adding a nitrification inhibitor.In conclusion,the inoculation of S2_8_1 enhanced the nitrification process in Italian ryegrass rhizosphere,stimulated the increase of NO_(3)^(−)content,and strengthened photosynthesis,and ultimately promoted the growth-promoting effect of Italian ryegrass.

关 键 词：氨氧化细菌 根际 土壤硝化作用 光合速率 再生 

分 类 号：S543.6[农业科学—作物学]