依赖流感聚合酶诱导报告系统评估流感病毒RNP兼容性  

Compatibility of influenza virus RNP assessed using polymerase inducible reporter system

作　　者：杨文广 李威 赵志艳 张森 陈月红 户义 姜涛[1,2] YANG Wenguang;LI Wei;ZHAO Zhiyan;ZHANG Sen;CHEN Yuehong;HU Yi;JIANG Tao(Institute of Microbiology and Epidemiology,Academy of Military Medical Sciences,Academy of Military Sciences,Beijing 100071,China;School of Basic Medical Sciences,Anhui Medical University,Hefei 230032,China)

机构地区：[1]军事科学院军事医学研究院微生物流行病研究所,北京100071 [2]安徽医科大学基础医学院,合肥230032

出　　处：《军事医学》2023年第8期584-589,共6页Military Medical Sciences

基　　金：病原微生物生物安全重点实验室面上项目(SKLPBS2120)。

摘　　要：目的探索建立不断变异的甲型流感病毒(IAV)的快速监测与评估模型,并通过该模型对与IAV复制水平密切相关的核糖核蛋白复合体(RNP)基因重组后的聚合酶活性和亚基相容性进行评估,最终对新发IAV进行监测。方法利用依赖流感病毒RNA聚合酶活性的荧光素酶报告系统,检测IAV不同亚型、相同亚型不同毒株间相应RNP重配体的聚合酶活性。结果病毒RNP的聚合酶活性受核蛋白单独重配影响不大,但其活性的高低与PB1⁃PB2、PB1⁃PA组合的来源密切相关;不同宿主来源的流感病毒PB1⁃PB2重配的RNP聚合酶活性下降显著。结论依赖流感病毒RNA聚合酶活性的荧光素酶报告系统可用于评估不同重配流感病毒RNP的兼容性。Objective To establish a rapid monitoring and assessment model for mutated influenza A virus(IAV),to evaluate the polymerase activity and subunit compatibility of the recombinant ribonucleoprotein(RNP)complex gene which is closely related to the replication level of IAV,and to monitor the new IAV.Methods Using the luciferase reporting system dependent on RNA polymerase activity of influenza virus,the polymerase activity of corresponding RNP heavy ligands among different subtypes of IAV and different strains of the same subtype was detected.Results The polymerase activity of virus RNP was not affected by the recombination of NP alone,but its activity was closely related to the source of the combination of PB1⁃PB2 and PB1⁃PA.The RNP polymerase activity of influenza virus PB1⁃PB2 from different host sources decreased significantly.Conclusion The luciferase reporting system,which depends on the activity of influenza virus RNA polymerase,can be used to assess the compatibility of different recombinant influenza virus RNPs.

关 键 词：甲型流感病毒 核糖核蛋白 聚合酶 基因重配 报告基因 

分 类 号：R373.13[医药卫生—病原生物学] Q933[医药卫生—基础医学]