基于脱细胞骨基质复合材料的制备方法及其理化性能研究  

Preparation methods and physicochemical properties of composite materials based on acellular bone matrix

作　　者：孙佳培 杜建 黄迅悟[2] 彭伟[1,2] SUN Jiapei;DU jian;HUANG Xunwu;PENG Wei(Joint Surgery Department,Orthopedics Medicine Department,the Fourth Medical Center,Chinese PLA General Hospital,Beijing 100142,China;The Eighth Medical Center,Chinese PLA General Hospital,Beijing 100091,China;Graduate School,Hebei North University,Zhangjiakou,Hebei 075132,China)

机构地区：[1]解放军总医院第四医学中心骨科医学部关节外科,北京100142 [2]解放军总医院第八医学中心,北京100091 [3]河北北方学院研究生院,河北张家口075132

出　　处：《军事医学》2023年第8期615-620,共6页Military Medical Sciences

基　　金：军队医学科技青年培育计划(孵化)项目(21QNPY117)。

摘　　要：目的物理混合制备脱细胞骨基质(ACBM)与磷酸钙/硫酸钙(CPC/CS)复合材料,以提升人工骨材料的可塑性并观察其理化特性。方法选取健康牛股骨干骺端松质骨制备ACBM,以ACBM为主体填充磷酸钙/半水硫酸钙(CPC/CSH),制备复合人工骨支架。设置ACBM占复合材料总体质量的10%、20%和30%3组,分别测量各组力学性能(弹性模量和抗压强度),选取其中最优力学性能组,采用X射线衍射(XRD)分析仪、扫描电镜(SEM)、透射电镜(TEM)、微量凯氏定氮法和液体置换法进一步分析其理化性能,并与同种异体骨和复合CPC/CSH进行力学性能比较。结果①ACBM不同占比复合材料中,20%ACBM复合人工骨材料具有最佳力学性能,能够承受最大应力923.81 N,最大变形4.92 mm,弹性模量(85.91±3.67)MPa,抗压强度(3.34±0.15)MPa,与其他两组相比差异具有统计学意义(P<0.001)。XRD显示,复合人工骨材料固化后主要是羟基磷灰石(HA)、CS和二水硫酸钙(CSD)等可吸收物质。扫描和透射电镜观察显示,材料孔径为(496.56±153.37)μm,内部孔隙相互贯通,连通性较好,缺损填充度高。材料蛋白质含量极低。液体置换法测量,孔隙率为(80.7±5.1)%。②力学性能测试,20%ACBM骨材料力学性能优于同种异体骨组和CPC/CSH组,差异有统计学意义(P<0.001)。结论20%ACBM复合人工骨材料具有较高的力学性能和孔隙率,可塑性强。Objective To improve the plasticity of artificial bone materials and observe their physical and chemical properties by physically mixing acellular bone matrix(ACBM)with calcium phosphate/calcium sulfate(CPC/CS).Methods The cancellous bone of a healthy bovine femoral head was selected to prepare ACBM,which was used as the main body to fill calcium phosphate/calcium sulfate hemihydrate(CPC/CSH)to prepare a composite artificial bone scaffold.ACBM was set to account for 10%,20%,and 30%of the total mass of the composite material respectively were measured before the mechanical properties(elastic modulus and compressive strength).The physical and chemical properties of the experimental group with the best mechanical properties were analyzed with an X⁃ray diffractometer(XRD),scanning electron microscope(SEM),transmission electron microscope(TEM),micro Kjeldahl nitrogen determination method,and liquid replacement method.The mechanical properties of the material with the best mechanical properties were compared with those of the allograft bone and composite CPC/CSH.Results①The 20%ACBM composite bone material had the best mechanical properties among the composite materials with different proportions of ACBM.It could withstand a maximum of 923.81 N,a maximum deformation of 4.92 mm,an elastic modulus of(85.91±3.67)MPa,and a compressive strength of(3.34±0.15)MPa(P<0.001).The XRD showed that this 20%ACBM composite artificial bone material could produce absorbable substances such as hydroxyapatite(HA),CS,and calcium sulfate dihydrate(CSD)after solidification.The pore size of the material observed by scanning and transmission electron microscopy was(496.56±153.37)μm,the internal pores were interconnected,and the connectivity and defect filling degree were high.This material had an extremely low protein content.The porosity was(80.7±5.1)%as was measured with the liquid displacement method.②The mechanical property test showed that the mechanical property of the 20%ACBM bone material was significantly better than that of

关 键 词：脱细胞骨基质 骨代用品 复合骨材料 硫酸钙 骨缺损 理化性能 

分 类 号：TB339[一般工业技术—材料科学与工程]