海绵共生木霉属真菌绿木霉中1个新的倍半萜类化合物  

A new sesquiterpenoid from Trichoderma virens,a marine sponge symbiotic Trichoderma sp.fungus

作　　者：张璐 刘辰鹏[1] 王瑞琪 焦伟华[2] 张小坡[1] ZHANG Lu;LIU Chen-peng;WANG Rui-qi;JIAO Wei-hua;ZHANG Xiao-po(School of Pharmaceutical Science,Hainan Medical University,Haikou 571199,China;State Key Laboratory of Oncogenes and Related Genes,Department of Pharmacy,Research Center for Marine Drugs,Ren Ji Hospital,School of Medicine,Shanghai Jiao Tong University,Shanghai 200127,China)

机构地区：[1]海南医学院药学院,海南海口571199 [2]上海交通大学医学院附属仁济医院药学部海洋药物研究中心(癌基因及相关基因国家重点实验室),上海200127

出　　处：《中草药》2023年第18期5840-5845,共6页Chinese Traditional and Herbal Drugs

基　　金：海南省科技重大专项(ZDKJ2021036)。

摘　　要：目的研究海绵共生真菌绿木霉(Trichoderma virens CMB-TN16)的次级代谢产物及其肿瘤细胞毒活性。方法利用硅胶柱色谱、半制备液相色谱等现代色谱技术方法进行分离纯化,结合核磁共振和质谱等现代谱学手段鉴定化合物结构,同时通过体外MTT法测定化合物的肿瘤细胞毒活性。结果从海绵共生真菌绿木霉醋酸乙酯提取物中分离得到7个倍半萜类化合物,分别鉴定为二绿木霉烯醇H(1)、二绿木霉烯醇D(2)、二绿木霉烯醇G(3)、黏埽霉酸(4)、(+)-氢化萜烯七脂酸(5)、(+)-3-O-乙酰萜烯七脂酸A(6)和木聚酸D(7)。化合物1体外对人大细胞肺癌NCI-H460细胞、人骨髓瘤NCI-H929细胞及人结肠癌SW620细胞的半数抑制浓度(half maximal inhibitory concentration,IC50)值分别为11.8、7.7和13.5μmol/L。结论化合物1为新化合物,对以上3株肿瘤细胞具有一定的细胞毒活性。Objective To study the secondary metabolites of a marine sponge symbiotic fungus Trichoderma virens CMB-TN16 and their antitumor cytotoxic activity.Methods The compounds were isolated and purified from EtOAc extract of CMB-TN16 by modern chromatography techniques,such as silica gel column chromatography,and semi-preparative high performance liquid chromatography,and their chemical structures were identified by NMR and MS spectrometry.Meanwhile,the antitumor cytotoxic activity of these compounds was evaluated by MTT method in vitro.Results Seven sesquiterpenoids were isolated from the EtOAc extract of CMB-TN16,and identified as divirensol H(1),divirensol D(2),divirensol G(3),gliocladic acid(4),hydroheptelidic acid(5),3-O-acetylheptelidic acid A(6)and xylaric acid D(7),respectively.The IC50 values of compound 1 against NCI-H460,NCI-H929 and SW620 were 11.8,7.7,and 13.5μmol/L,respectively.Conclusion Compound 1 is a new compound,and have exhibited moderate cytotoxic activities against three tumor cell lines.

关 键 词：海绵 绿木霉 倍半萜 抗肿瘤 细胞毒活性 二绿木霉烯醇H (+)-3-O-乙酰萜烯七脂酸 

分 类 号：R284.1[医药卫生—中药学]