基于“化学物质组-血中移行成分-药动学”的蒙药暖宫七味丸质量标志物研究  

Quality markers of Mongolian medicine Nuangong Qiwei Pill based on“chemical composition-blood migration component-pharmacokinetics”

作　　者：张晨晨 张东旭 张玉凤 张爱杰 董世奇 韩峰 邢界红 樊慧蓉[2] 刘昌孝[5] ZHANG Chen-chen;ZHANG Dong-xu;ZHANG Yu-feng;ZHANG Ai-jie;DONG Shi-qi;HAN Feng;XING Jie-hong;FAN Hui-rong;LIU Xiao-chang(Tianjin university of Traditional Chinese Medicine,Tianjin 301617,China;Institute of Radiation Medicine,Chinese Academy of Medical Sciences,Tianjin 300192,China;Inner Mongolia Institute for Drug Control,Hohhot 010020,China;Inner Mongolia Mongolian Medicine Co.,Ltd.,Tongliao 028000,China;Tianjin Institute of Pharmaceutical Research,Tianjin 300462,China)

机构地区：[1]天津中医药大学,天津301617 [2]中国医学科学院放射医学研究所,天津300192 [3]内蒙古自治区药品检验研究院,内蒙古呼和浩特010020 [4]内蒙古蒙药股份有限公司,内蒙古通辽028000 [5]天津药物研究院,天津300462

出　　处：《中草药》2023年第18期5855-5866,共12页Chinese Traditional and Herbal Drugs

基　　金：内蒙古自治区科技计划项目(2020GG0199);国家重点研发计划项目(2018YFC1708203)。

摘　　要：目的研究蒙药暖宫七味丸的化学物质组、血中移行成分及药动学,筛选出暖宫七味丸潜在的质量标志物。方法综合运用UPLC-Triple-TOF-MS/MS、GC-MS技术结合数据库及对照品对比,鉴别暖宫七味丸的化学物质组、血中移行成分;使用UPLC-Q-TRAP-MS/MS研究其体内药动学特征。结果采用UPLC-Q-TOF-MS/MS鉴别出暖宫七味丸中的76个化学成分,采用GC-MS鉴别出35个化合物。经大鼠ig给药后鉴别出19个吸收进入血中的原型成分,其中,沉香四醇、绿原酸、科罗索酸表现出良好的可测性,且具有明显的药动学特征。结论沉香四醇、绿原酸、科罗索酸在暖宫七味丸的化学物质组-血中移行成分-药动学研究中具有良好的可测性和可追溯性,可作为暖宫七味丸潜在的质量标志物。Objective To study the chemical composition,blood migration components and pharmacokinetics and screen the potential quality markers of Nuangong Qiwei Pill(暖宫七味丸),a Mongolian medicine.Methods The UPLC-Triple-TOF-MS/MS and GC-MS technologies were used in combination with the database and the comparison of standard products to identify the chemical composition and blood migration components of Nuangong Qiwei Pill;UPLC-Q-TRAP-MS/MS was used to study its pharmacokinetics in vivo.Results A total of 76 chemical components were identified by UPLC-Q-TOF-MS/MS and 35 compounds were identified by GC-MS.After intragastric administration in rats,19 prototype components that were absorbed into the blood were identified.Among them,agarotetrol,chlorogenic acid and corosolic acid showed good testability and obvious pharmacokinetic characteristics.Conclusion Agarotetrol,chlorogenic acid and corosolic acid may be potential quality markers of Nuangong Qiwei Pill due to its good measurability and traceability in chemical composition-blood migration component-pharmacokinetics analysis.

关 键 词：暖宫七味丸 质量标志物 化学物质组 血中移行成分 药动学 沉香四醇 绿原酸 科罗索酸 

分 类 号：R284.1[医药卫生—中药学]