肠道菌群移植对大鼠肾脏中草酸钙结晶的抑制作用  

Inhibitory effect of fecal microbiota transplantation on renal calcium oxalate crystal deposition in rats

作　　者：王演 高磊 孙进波 刘振宇 周宇 李超圣 潘铁军 Wang Yan;Gao Lei;Sun Jinbo;Liu Zhenyu;Zhou Yu;Li Chaosheng;Pan Tiejun(Department of Urology,General Hospital of Central Theater Command,Wuhan 430000,China)

机构地区：[1]中部战区总医院泌尿外科,武汉430000

出　　处：《中华泌尿外科杂志》2023年第9期682-689,共8页Chinese Journal of Urology

基　　金：国家自然科学基金(81502210);湖北省卫生计生科研基金(WJ2015MB047)。

摘　　要：目的探讨肠道菌群移植(FMT)对高草酸诱导的SD大鼠肾草酸钙结晶形成的影响。方法6只雄性豚鼠给予正常豚鼠饲料喂养1个月,而后给予含5%草酸的饲料喂养,收集5%草酸饮食前及第14天的豚鼠粪便,分别记为豚鼠正常饮食(GSC)组和豚鼠高草酸饮食(GOD)组。将24只雄性SD大鼠随机分为正常饮食(SC)组、高草酸饮食(OD)+磷酸缓冲盐溶液(PBS)灌胃组(OD+PBS组).OD+肠道菌群移植(FMT)组和SC+FMT组。其中,SC组和SC+FMT组采用正常大鼠饲料喂养;OD+PBS组和OD+FMT组采用含5%草酸的饲料喂养;OD+FMT组和SC+FMT组大鼠给予G0D组的豚鼠粪便滤液灌胃,连续灌胃7 d。收集各组大鼠的24 h尿液及粪便。对粪便标本的16S rRNA基因进行测疗,检测大鼠及豚鼠的肠道菌群。采用高效液相色谱法检测大鼠尿草酸含量。对各组大鼠及其肾脏称重,计算肾脏指数。对大鼠肾脏组织行HE染色观察组织学形态变化,行Pizzolato染色检测肾组织内草酸钙结晶沉积情况。结果与GSC组相比,GOD组肠道中Muribaculaceae科、双歧杆菌科等共11个科细菌相对丰度明显增加。OD+PBS组较SC组大鼠肠道菌群的微生物多样性降低,OD+FMT组较OD+PBS组大鼠肠道菌群的微生物多样性有所恢复。SC+FMT组大鼠肠道菌群偏离了SC组,与GOD组菌群相似度增加。在OD+FMT组中,Muribaculaceae科、丹毒丝菌科及双歧杆菌科等共18个科的细菌显著富集,FMT激活了以Muribaculaceae科细菌为代表的肠道微生物网络。与SC组相比,OD+PBS组大鼠的肾脏指数明显增加(6.12±0.53与7.63±0.67,P<0.05),而OD+FMT组较OD+PBS组肾脏指数显著降低(6.53±0.64与7.63±0.67,P<0.05)。SC组0D+PBS组及OD+FMT组大鼠的尿草酸含量分别为(0.61±0.05)、(0.89±0.04).(0.72±0.04)μumol/ml。SC组大鼠肾脏中未见草酸钙结晶(0分),OD+PBS组中检测到较多的草酸钙结晶[(4.83±0.41)分],OD+FMT组草酸钙结晶评分[(3.17±0.75)分]较OD+PBS组更低(P<0.01)。结论FMT激活了Objective To explore the effect of fecal microbiota transplantation(FMT)on the formation of renal calcium oxalate crystals in SD rats induced by oxalate mixed diet.Methods Six male guinea pigs were fed with standard guinea pig chow for 1 month and then given a 5%oxalate diet for 14 d.The guinea pigs on the standard chow were labeled as the standard chow guinea pig(CSC group)and those on the high oxalate diet for 14 d were labeled as the guinea pig group on the high oxalate diet(COD group).The feces of guinea pigs in the CSC and COD groups were collected using metabolic cages.Twenty-four male SD rats were randomly divided into standard chow(SC)group,oxalate diet(OD)+phosphate buffered saline gavage group(OD+PBS group),OD+FMT group and SC+FMT group.Among them,the SC group and SC+FMT group were fed with standard chow.The OD+PBS group and OD+FMT group were fed with 5%oxalate content chow.The OD+FMT and SC+FMT groups were given GOD group guinea pig fecal filtrate gavage for 7 days.The 24 h urine and feces of rats in each group were collected,and the intestinal microbiota of rats and guinea pigs were detected by 16sRNA detection.The urinary oxalate excretion was detected by high performance liquid chromatography.The rats and kidneys were weighed and the renal index was calculated.HE staining was used to observe the histological morphological changes of rat kidney tissue,the calcium oxalate crystal deposition in renal tissues was detected by Pizzolato staining.Results The relative abundance of bacteria from a total of 1l families,including Muribaculaceae family and Bifidobacteriaceae family,was significantly increased in the intestinal tract of guinea pigs(COD)from the high oxalate diet group compared to guinea pigs(GSC)from the standard chow group.The microbial diversity of the intestinal microbiota of the rats in the OD+PBS group was reduced compared to the SC group,and the microbial diversity of the intestinal microbiota of the rats in the OD+FMT group was restored compared to the OD+PBS group.When given a standard c

关 键 词：动物实验 肠道菌群移植 草酸钙结石 草酸降解菌 肠道微生物网络 

分 类 号：R692[医药卫生—泌尿科学]