中度寻常性银屑病患者血浆对Jurkat细胞作用的研究  

Effect of plasma from the patients with moderate psoriasis vulgaris on Jurkat cells

作　　者：邹朋 李俊琴[2] 王泽洪 李新华[2] ZOU Peng;LI Junqin;WANG Zehong;LI Xinhua(Department of Microbiology and Immunology,School of Basic Medical Sciences,Shanxi Medical University,Jinzhong 030619,China;Laboratory of Dermatology,Taiyuan Central Hospital,Shanxi Medical University,Taiyuan 030009,China)

机构地区：[1]山西医科大学基础医学院微生物与免疫学教研室,山西晋中030619 [2]山西医科大学附属太原中心医院皮肤科实验室,山西太原030009

出　　处：《临床皮肤科杂志》2023年第10期590-593,共4页Journal of Clinical Dermatology

基　　金：国家自然科学基金(81401360)资助项目。

摘　　要：目的:探究中度寻常性银屑病患者血浆微环境对Jurkat细胞炎症因子分泌、增殖及凋亡活性的影响。方法:分别采用中度寻常性银屑病患者(试验组)和正常人(对照组)的血浆培养Jurkat细胞,48 h后采用实时荧光定量聚合酶链式反应(RTqPCR)检测Jurkat细胞分泌白细胞介素(IL)-8、IL-10、增殖核抗原(Ki-67)、细胞死亡受体1(Fas)、Fas配体(FasL)、胱天蛋白酶(Caspase)-3及Caspase-8的mRNA相对表达水平。结果:RT-qPCR结果显示与对照组相比,试验组Jurkat细胞表达促炎因子IL-8水平增高,抑炎因子IL-10水平降低(P<0.05);Fas、FasL、Caspase-3及Caspase-8表达水平均显著上调(P<0.05);而Ki-67表达差异无统计学意义(P>0.05)。Spearman相关性分析显示,试验组中高表达的IL-8水平与胞内Fas(r=0.72,P<0.05)、FasL(r=0.54,P<0.05)、Caspase-3(r=0.62,P<0.05)、Caspase-8(r=0.69,P<0.05)mRNA表达均呈正相关;低表达的IL-10水平与胞内Fas(r=-0.40,P<0.05)、Caspase-3(r=-0.46,P<0.05)、Caspase-8(r=-0.62,P<0.05)mRNA表达均呈负相关。结论:银屑病患者血浆可诱导Jurkat细胞IL-8及IL-10表达异常,并增强Fas/FasL凋亡途径;且IL-8与Fas/FasL凋亡呈正相关,IL-10与Fas/FasL凋亡呈负相关,提示银屑病血浆微环境异常可能是T细胞活性异常的形成机制之一。Objective:To investigate the effect of the plasma microenvironment of patients with moderate psoriasis vulgaris on the secretion of inflammatory cytokines,proliferation and apoptosis of Jurkat cells.Methods:Jurkat cells were cultured using fresh plasma from moderate psoriasis and normal human,respectively.The mRNA expression levels of inflammatory cytokines(IL-8,IL-10),proliferation indicators(Ki-67),and apoptosis indicators(Fas,Fas ligand,Caspase-3,Caspase-8)were measured in Jurkat cells after 48 h incubation.Results:RT-qPCR results showed that compared to the control group,the psoriasis group exhibited increased levels of the pro-inflammatory cytokine IL-8 and decreased levels of the anti-inflammatory cytokine IL-10 in Jurkat cells stimulated with psoriasis plasma(P<0.05).The expression levels of Fas,FasL,Caspase-3,and Caspase-8were all increased(P<0.05),while there was no statistically significant difference in Ki-67 expression(P<0.05)in the psoriasis group,compared with control group.Spearman correlation analysis revealed that in the psoriasis group,the high expression of IL-8 was positively correlated with the intracellular mRNA expression of Fas(r=0.72,P<0.05),FasL(r=0.54,P<0.05),Caspase-3(r=0.62,P<0.05),and Caspase-8(r=0.69,P<0.05).Conversely,the low expression of IL-10 was negatively correlated with the intracellular mRNA expression of Fas(r=-0.40,P<0.05),Caspase-3(r=-0.46,P<0.05),and Caspase-8(r=-0.62,P<0.05).Conclusion:Psoriasis plasma can induce abnormal expression of IL-8 and IL-10 in Jurkat cells and enhance the Fas/FasL apoptosis pathway.Moreover,there is a positive correlation between the expression level of IL-8 and Fas/FasL apoptosis,and a negative correlation between the expression of IL-10 and Fas/FasL apoptosis.Abnormal plasma microenvironment in psoriasis may be one of the mechanisms promoting T cell dysfunction.

关 键 词：银屑病 血浆微环境 JURKAT细胞 炎症 Fas/FasL凋亡途径 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]