荧光标记技术在新型农用化合物创制中的应用现状及进展  

Application Status and Progress of Fluorescent Labeling Technology in the Creation of Novel Agrocompounds

作　　者：文鼎 张亚[1] 刘双清[1] 王翀[2] 廖晓兰[1] WEN Ding;ZHANG Ya;LIU Shuangqing;WANG Chong;LIAO Xiaolan(College of Plant Protection,Hunan Agricultural University,Changsha 410128,China;College of Bioscience and Biotechnology,Hunan Agricultural University,Changsha 410128,China)

机构地区：[1]湖南农业大学植物保护学院,湖南长沙410128 [2]湖南农业大学生物科技学院,湖南长沙410128

出　　处：《河南农业科学》2023年第9期1-13,共13页Journal of Henan Agricultural Sciences

基　　金：国家自然科学基金面上项目(32072473);湖南省教育厅科学研究项目(21B0199)。

摘　　要：荧光标记技术广泛应用于现代社会的各个方面,但其在农用化合物创制领域的应用鲜见综述。基于近10 a国内外农用化合物创制领域的荧光标记技术相关文献,系统总结了荧光标记技术中荧光染料的种类,综合介绍了荧光探针技术和免疫荧光技术在新型农用化合物创制领域的应用现状及进展,分析了目前荧光标记技术在新型农用化合物创制领域存在的局限与不足,并对未来荧光标记技术的发展趋势进行了展望。Fluorescent labeling technology(FLT)is widely used in all aspects of the modern society,but its application in the field of agrocompound creation has rarely been reviewed.Based on the domestic and foreign literature on FLT in the field of agrocompound creation in the past 10 years,the kinds of fluorescent dyes in FLT was introduced,the application situation and progress of fluorescence probe technology and immunofluorescence technology in new agrocompound creation field were summarized,the limitations and deficiencies of FLT in the field of agrocompound creation were analyzed,and the development trend of FLT in the future was prospected.

关 键 词：荧光标记 农用化合物创制 荧光染料 荧光探针 免疫荧光 

分 类 号：S482[农业科学—农药学] F767.2[农业科学—植物保护] O657.3[经济管理—产业经济]