植物激素与螯合剂复合处理对镉胁迫下马齿苋发育和修复效果的影响  

Effects of Phytohormones and Chelating Agents on the Development and Remediation Effect of Portulaca oleracea L.under Cadmium Stress

作　　者：郭梦露 李建奇[1] 鲁豫 GUO Menglu;LI Jianqi;LU Yu(College of Agricultural and Biological Engineering,Heze University/Key Laboratory of Plant Biology Heze 274015,China)

机构地区：[1]菏泽学院农业与生物工程学院/植物生物学重点实验室,山东菏泽274015

出　　处：《河南农业科学》2023年第9期102-109,共8页Journal of Henan Agricultural Sciences

基　　金：山东省自然科学基金项目(ZR2022MC203);山东省高校科技计划项目(J14LE54);菏泽学院植物生物学重点实验室资助项目。

摘　　要：为研究植物激素和螯合剂EDTA复合处理对镉(Cd)胁迫下马齿苋发育和修复效果的影响,设置3个Cd胁迫下的植物激素和EDTA复合处理[FI:20mg/LCd+1.5mg/LEDTA+100mg/LIAA(生长素);FG:20mg/L Cd+1.5 mg/LEDTA+500 mg/LGA(赤霉素);FS:20 mg/L Cd+1.5mg/LEDTA+100mg/L SA(水杨酸)],以蒸馏水为空白对照(CK)、以20mg/LCd处理为Cd胁迫对照(Cd),分别对马齿苋种子进行浸种,测定种子发芽势、发芽率,幼苗苗高、主根长、鲜质量,以及幼苗各部分Cd积累量、转运系数。结果表明,与Cd胁迫对照相比,3个复合处理的种子发芽势和发芽率显著增高,其中,FG处理发芽势和发芽率增幅最大,分别增加19.24%、25.31%;苗高、主根长、鲜质量均显著增加,其中,FG处理幼苗苗高、鲜质量增幅最大,分别增加47.95%、35.91%;根部和地上部对Cd的积累量和转运系数显著增加,其中,FG处理马齿苋幼苗根部、地上部Cd积累量及转运系数均为最大,分别为Cd胁迫对照的2.31、2.94、2.33倍。综上,植物激素与EDTA复合处理可增大Cd胁迫下马齿苋种子发芽势、发芽率,增加幼苗苗高、主根长、鲜质量,提高Cd的积累量和转运系数,其中,GA与EDTA复合处理效果最佳。To study the effects of phytohormones and chelating agent EDTA combined treatment on the development and repair effect of Portulaca oleracea L.under cadmium(Cd)stress,three phytohormone and EDTA combined treatments were set under cadmium stress(FI:20 mg/L Cd+1.5 mg/L EDTA+100mg/LIAA;FG:20 mg/L Cd+1.5mg/LEDTA+500mg/LGA;FS:20 mg/L Cd+1.5mg/LEDTA+100 mg/L SA),with distilled water as blank control(CK),and 20 mg/L Cd treatment as Cd stress control(Cd),and seeds of Portulaca oleracea L.were soaked in dfferent liquids.The seed germination potential,germination rate,seedling height,taproot length,fresh weight,as well as the accumulation and transport coefficient of Cd in various parts of the seedlings were measured.The results showed that compared with Cd stress control,the seed germination potential and germination rate of the three composite treatments were significantly increased.Among them,the FG treatment had the largest increase in germination potential and germination rate,with an increases of 19.24%and 25.31%,respectively.The seedling height,taproot length,and fresh weight all significantly increased,among them the FG treatment had the largest increase in seedling height and fresh weight,with increases of 47.95%and 35.91%compared with Cd stress control,respectively.The accumulation and translocation factor of Cd in the roots and shoots of Portulaca oleracea L.seedlings significantly increased.Among them,the accumulation and translocation factor of Cd in the shoots and roots of Portulaca oleracea L.seedlings treated with FG were the highest,2.31,2.94,and 2.33 times of those treated with Cd stress control,respectively.In summary,the combined treatment of phytohormones and EDTA can increase the germination potential and percentage of Portulaca oleracea L.seeds under Cd stress,increase the seedling height,taproot length,fresh weight,and increase the accumulation and translocation factor of Cd.Among them,the combined treatment of GA and EDTA has the best effect.

关 键 词：马齿苋 镉胁迫 植物激素 螯合剂 种子萌发 幼苗发育 积累量 转运系数 

分 类 号：S567[农业科学—中草药栽培]