基于网络药理学分析白头翁治疗奶牛乳腺炎的作用机制  

Analysis on the mechanism of Pulsatilla chinensis in treating dairy cowmastitis based on network pharmacology

作　　者：李雄婕 严作廷[2] 曹随忠[1] LI Xiongjie;YAN Zuoting;CAO Suizhong(College of Veterinary Medicine,Sichuan Agricultural University,Chengdu Sichuan 611130,China;Lanzhou Institute of Husbandry and Pharmaceutical Sciences of CAAS,Lanzhou Gansu 730050,China)

机构地区：[1]四川农业大学动物医学院,四川成都611130 [2]中国农业科学院兰州畜牧与兽药研究所,甘肃兰州730050

出　　处：《中兽医医药杂志》2023年第5期51-56,共6页Journal of Traditional Chinese Veterinary Medicine

基　　金：甘肃省科技厅重点研发计划项目(20YF8NA029);四川省科技厅科技计划项目(2019YJ0650)。

摘　　要：利用网络药理学研究方法探究白头翁治疗奶牛乳腺炎的活性成分、作用靶点及其基因功能、信号通路的机制。检索白头翁的所有化学成分及作用靶点,构建药物-疾病-靶点网络图、蛋白质-蛋白质相互作用(PPI)网络图、靶点-通路气泡图,综合数据信息分析得出白头翁治疗奶牛乳腺炎的作用机制。结合数据结果推测,白头翁中以异鼠李素为主的各活性成分通过影响表皮生长因子受体(EGFR)、肝X受体(LXRs)、雌激素受体1(ESR1)、Toll样受体等关键基因靶点,对促分裂原活化蛋白激酶(MARK)信号通络、蛋白酪氨酸激酶(JAK)-转录活化因子(STAT)信号通路、磷酯酰肌醇-3激酶(PI3K)/蛋白激酶B(AKT)、核因子κB(NF-κB)信号轴等通路及T细胞膜糖蛋白分子CD4、细胞色素P450、一氧化氮合酶NOS2等基因进行靶点调控,从而发挥降低炎症反应、调节乳腺上皮细胞生长的作用,达到对乳腺炎的治疗效果。通过网络药理学研究方法探索白头翁活性成分、药物和疾病影响靶标及其映射关系,分析药物治疗疾病的影响通路,为奶牛乳腺炎的治疗提供参考。Using network pharmacological research method,this experiment tried to reveal the active components,targets,gene functions and signal pathway mechanism of Pulsatilla chinensis in treating mastitis of dairy cows.The mechanism of Pulsatilla chinensis in treating cow mastitis was described by comprehensive data analysis of the chemical components and target sites of Pulsatilla chinensis,drug-disease-target network map,protein-protein interaction(PPI)network map and target-pathway bubble map.Based on the data results,the active components of Pulsatilla chinensis,mainly isorhamnetin,affect key gene targets such as epidermal growth factor receptor(EGFR),liver X receptor(LXRs),estrogen receptor 1(ESR1)and Toll-like receptor to stimulate mitogen-activated protein kinase(MARK)signaling pathways,protein tyrosine kinase(JAK)-transcriptional activation factor(STAT)signaling pathways,phosphoinositol-3 kinase(PI3K)/protein kinase B(AKT),nuclear factorκB(NF-κB)signaling axis,and gene target of T cell membrane glycoprotein molecules CD4,cytochrome P450,nitric oxide synthase NOS2 and other genes.These actions play the role effects in reducing inflammation and regulating the growth of mammary epithelial cells to treat mastitis.The results can provide reference for the treatment of cow mastitis.

关 键 词：白头翁 奶牛乳腺炎 蛋白质-蛋白质相互作用(PPI)网络图 KEGG信号通路 

分 类 号：S853.74[农业科学—临床兽医学]