HPLC法同时测定西藏凹乳芹根中4种成分的含量  

Simultaneous determination of four components in Vicatia thibetica de Boiss.by HPLC

作　　者：李阳[1,2] 余晓晖 杜弢[1] 朱昕宇[1] 郭玫 LI Yang;YU Xiaohui;DU Tao;ZHU Xinyu;GUO Mei(Gansu University of Chinese Medicine,Lanzhou Gansu 730000,China;Key Laboratory of Chemistry and Quality for Traditional Chinese Medicine(TCM)of College in Gansu Province,Lanzhou Gansu 730000,China)

机构地区：[1]甘肃中医药大学,甘肃兰州730000 [2]甘肃省高校中(藏)药化学与质量研究重点实验室,甘肃兰州730000

出　　处：《中兽医医药杂志》2023年第5期78-80,共3页Journal of Traditional Chinese Veterinary Medicine

基　　金：甘肃省中医药科研项目(Gzk-2016-58);甘肃省高校中(藏)药化学与质量研究省级重点实验室开放基金项目(zzy-2019-05)。

摘　　要：建立同时测定西藏凹乳芹中伞形花内酯、佛手柑内酯、芹菜素以及阿魏酸等含量的方法。采用SinoChrom ODS-BP柱(250.0 mm×4.6 mm,5μm);检测波长320 nm;柱温30℃;流动相为0.05%磷酸-甲醇,进行梯度洗脱;流速为1 mL/min。结果显示,伞形花内酯、佛手柑内酯在0.0050~0.5000 mg/mL范围内,阿魏酸在0.0100~0.5000 mg/mL范围内,芹菜素在0.0025~0.5000 mg/mL范围内有良好的线性关系,平均回收率分别为102.00%、96.00%、96.58%、104.40%。本试验建立的方法可行性较高,可以用于同时测定西藏凹乳芹中伞形花内酯、佛手柑内酯、芹菜素以及阿魏酸4种成分的含量。The objective was to establish an HPLC method for simultaneous determination of umbelliferone,bergapten,apigenin and ferulic acid in Vicatia thibetica de Boiss.The chromatographic column was SinoChrom ODS-BP(250.0 mm×4.6 mm,5μm);the mobile phase was 0.05%phosphoric acid-methanol,gradient elution with flow rate of 1.0 mL/min.The detection wavelength was 320 nm,column temperature was 30℃.The results showed that the linear range of umbelliferone and bergapten was 0.0050-0.5000 mg/mL,that of ferulic acid and apigenin was 0.0100-0.5000 mg/mL and 0.0025-0.5000 mg/mL.The average recoveries were 102.00%,96.00%,96.58%and 104.40%,respectively.The method is highly feasible and can be used to determine the content of these four components simultaneously.

关 键 词：HPLC法 西藏凹乳芹 含量测定 

分 类 号：S853.7[农业科学—临床兽医学]