苹果PIN家族基因鉴定及在不定根形成中的表达分析  

Identification and expression analysis of PIN gene family during adventitious root formation in apple

作　　者：方森 宋雪莲 韩轩轩 宋春晖 焦健 王苗苗 宋尚伟[1] 郑先波[1] 白团辉[1] FANG Sen;SONG Xuelian;HAN Xuanxuan;SONG Chunhui;JIAO Jian;WANG Miaomiao;SONG Shangwei;ZHENG Xianbo;BAI Tuanhui(College of Horticulture,Henan Agricultural University,Zhengzhou 450046,Henan,China)

机构地区：[1]河南农业大学园艺学院,郑州450046

出　　处：《果树学报》2023年第9期1789-1799,共11页Journal of Fruit Science

基　　金：国家重点研发计划(2019YFD1000100、2018YFD1000300);国家自然科学基金(31872058);河南省科技攻关项目(222102110041);河南省大宗水果产业技术体系(ARRS-22-09-Z2)。

摘　　要：【目的】探究苹果PIN全基因组特征及在不定根形成过程中的表达分析,以便更深入地了解其结构特点与潜在功能,为研究不定根形成的分子机制奠定基础。【方法】利用苹果基因组数据库GDR和HMMER对苹果PIN基因家族成员进行鉴定并编号,用MEGA、TBtools和MEME等生物信息学软件对其家族成员的基因定位、系统进化关系、基因结构、保守motif基序和蛋白保守结构域等进行分析,并采用qRT-PCR方法对PIN基因家族成员在不定根形成中的表达模式进行分析。【结果】苹果PIN基因家族有13个成员,分布于基因组8条染色体上,氨基酸数目在357~660个之间,蛋白质分子质量为38.84~71.61 ku,等电点在6.93~9.35之间。启动子作用元件分析表明,13个PIN启动子上含有响应激素、生长发育和逆境相关的顺式作用元件,其中有8个PIN基因含有生长素响应作用元件,暗示他们可能参与生长素调控不定根形成。转录组分析显示,13个PIN家族成员中,有6个基因(MdPIN3、MdPIN4、MdPIN6、MdPIN7、Md-PIN11和MdPIN12)在吲哚丁酸(IBA)处理下表达上调。进一步通过qRT-PCR分析,发现MdPIN3、MdPIN4、MdPIN6和MdPIN11在IBA处理下都有明显上升趋势,其中MdPIN4表达量变化最大。【结论】通过对苹果全基因组进行分析,共鉴定到13个PIN基因,分布在8条染色体上,通过转录组和qRT-PCR分析,推测MdPIN4可能在调控苹果砧木不定根形成中起着重要作用。【Objective】Apple(Malus×domestica Borkh.)is one of the most economically important tree fruits in China,and the intensive cultivation with dwarfing rootstock is popular in the world.The use of dwarfing rootstocks is the main way to realize the intensive cultivation of apple.The formation of adventitious roots is one of the key steps in the vegetative propagation of dwarfing rootstocks.The auxin transporter PIN-FORMED(PIN)protein is a typical transmembrane transport protein,which plays an important role in adventitious root formation by participating in the polar transport of auxin in plants.In this study,the characteristics of the apple PIN gene family at the whole genome and its expression in the process of adventitious root formation were investigated,so as to better understand its structural characteristics and potential functions,and lay a foundation for studying the molecular mechanism of adventitious root formation.【Methods】The apple PIN gene family members were identified using the apple genome database GDR and HMMER.The gene mapping,phylogenetic relationship,gene structure,conserved motif analysis and protein conserved domain of the PIN gene family members were analyzed using bioinformatics software such as MEGA,TBtools and MEME.qRT-PCR was used to analyze the expression pattern of the PIN gene family members in adventitious root formation.【Results】There were 13 members of the apple PIN gene family,and they were distributed on 8 chromosomes.The amino acid lengths ranged from 357 to 660 aa,the molecular weights of protein ranged from 38.84 to 71.61 ku,and the isoelectric points ranged from 6.93 to 9.35.The sequence analysis showed the 13 PIN genes were unevenly distributed on 8 chromosomes of apple(Chr 4,Chr 6,Chr 9,Chr 10,Chr 12,Chr 13,Chr 14 and Chr 16),among which there were two PIN genes on Chr 4,12,13,14 and 16 respectively,there was only one PIN gene on Chr 6,9 and 10.The collinear analysis showed that there was segment replication between the MdPIN2 and the MdPIN7,the MdPIN3 and the MdPI

关 键 词：苹果 PIN 全基因组鉴定 不定根形成 基因表达分析 

分 类 号：S661.1[农业科学—果树学]