深层含氟地下水微生物群落组成及环境响应特征  

Characteristics of Microbial Community Composition and Environmental Response in Deep Fluorinated Groundwater

作　　者：张怀胜 王梦园 蔡五田[1] 边超[1] 刘金巍[1] Zhang Huaisheng;Wang Mengyuan;Cai Wutian;Bian Chao;Liu Jinwei(Center for Hydrogeology and Environmental Geology Survey,CGS,Baoding 071051,China;Hubei Academy of Geological Sciences,Wuhan 430034,China)

机构地区：[1]中国地质调查局水文地质环境地质调查中心,河北保定071051 [2]湖北省地质科学研究院,湖北武汉430034

出　　处：《地球科学》2023年第9期3466-3479,共14页Earth Science

基　　金：国家重点研发计划(No.2019YFC1804804);国家基础资源调查专项课题(No.2017FY100401);国家水体污染控制与治理科技重大专项子课题(No.2018ZX07109⁃001⁃02).

摘　　要：在特殊地质体中开展地质微生物研究具有重要价值,但在深层含氟地下水环境中开展的相关研究尚有不足.为此,选取衡水市桃城区第三含水组并采集地下水水源井样品9个,进行了水化学分析和微生物16s RNA基因V4-V5区域测序.结果表明,研究区深层地下水偏碱性,TDS相对偏低,F^(‒)的平均含量为1.01 mg/L,水化学类型整体表现为Cl·SO_(4)-Na与HCO_(3)·Cl·SO_(4)-Na型.F^(‒)含量对地下水微生物的丰度与多样性影响较为明显,高氟与低氟地下水特有菌属数量分别为39个和126个.地下水在门水平上以变形菌门(Proteobacteria)最为优势(47.67%~76.96%),属水平类群分散度较高,无明显优势菌属.整体上看,研究区地下水中菌群的组成对水化学过程响应灵敏且关系密切,NO_(3)^(‒)、F^(‒)、DO与取样深度是影响微生物组成结构的主要因子,其中DO也是研究区地下水微生物丰度水平的重要保障.Conducting microbiological research in unique geological formations is of significant value,yet related studies within deep fluorine-rich groundwater environments remain insufficient.To dress this gap,9 groundwater source well samples were collected from the 3rd hydrous group of Taocheng District of Hengshui City,China,and the water chemistry analysis and microbial 16S RNA gene V4-V5 region sequencing were performed.The results showed that the deep groundwater in the studied area was slightly alkaline with relatively low TDS and the average content of F^(‒)was 1.01 mg/L.The overall water chemistry types were characterized as Cl·SO_(4)-Na and HCO_(3)·Cl·SO_(4)-Na types.The influence of F^(‒)content on the abundance and diversity of groundwater microorganisms was obvious,with 39 and 126 genera unique to high vs.low fluoride groundwater,respectively.Groundwater was dominated by Proteobacteria at the phylum level(47.67%-76.96%),with a high dispersion of taxa at the genus level and no obvious dominant genera.On the whole,the composition of the bacteria in groundwater of the study area is sensitive and strongly correlates with water chemical processes.NO_(3)^(‒),F^(‒),DO and sampling depth are predominantly influence microbial composition,and DO also critically supports the microbial abundance levels in groundwater in the study area.

关 键 词：深层地下水 微生物 生物化学 环境响应特征 地下水 

分 类 号：P641[天文地球—地质矿产勘探]