蛇床子素对丙泊酚诱导麻醉大鼠认知功能的影响与机制  

Mechanism and effects of osthole on cognitive function in rats induced by propofol

作　　者：周超瑞 顾健坤 陈娜 李明娟[3] ZHOU Chaorui;GU Jiankun;CHEN Na;LI Mingjuan(Graduate school,Zhejiang University of Chinese Medicine,Hangzhou 310053,Zhejiang,China;Department of Anesthesiology,China Coast Guard Hospital of the People’s Armed Police Force,Jiaxing 314000,Zhejiang,China;Jiaxing University Medical College,Jiaxing 314001,Zhejiang,China)

机构地区：[1]浙江中医药大学研究生院,浙江杭州310053 [2]中国人民武装警察部队海警总队医院麻醉科,浙江嘉兴314000 [3]嘉兴学院医学院,浙江嘉兴314001

出　　处：《中国现代医生》2023年第27期84-88,共5页China Modern Doctor

摘　　要：目的 研究观察蛇床子素(osthole,OST)对丙泊酚诱导麻醉大鼠认知功能的影响,并探讨其作用机制。方法 将雄性SD大鼠分为空白组、模型组、治疗组。大鼠先行腹腔注射丙泊酚(100mg/kg)一次造模,治疗组自麻醉后灌胃给药OST(10mg/kg),每天1次,连续给药14d。Morris水迷宫实验观察大鼠的空间学习及记忆能力,Western blot检测炎症因子白细胞介素(interleukin,IL)-1β和肿瘤坏死因子(tumor necrosis factor,TNF)-α的蛋白表达。结果 Morris水迷宫结果显示,与空白组相比,模型组大鼠的记忆能力下降,逃避潜伏期明显延长;与模型组相比,治疗组大鼠的记忆能力得到改善,逃避潜伏期明显缩短。Western blot结果分析发现,与空白组大鼠相比,模型组大鼠海马组织中IL-1β和TNF-α蛋白表达均增加,给予OST灌胃治疗后,治疗组大鼠海马组织中上述蛋白表达均降低。结论 OST可改善丙泊酚诱导麻醉大鼠引起的认知功能障碍,其作用机制可能与降低大鼠海马组织中炎症因子IL-1β和TNF-α的表达有关。Objective To observe the effect of osthole(OST)on cognitive function in rats induced by propofol and to explore its mechanism.Methods Male SD rats were divided into blank group,model group and treatment group.The rats were first modeled by intraperitoneal injection of propofol(100mg/kg)once,and the treatment group was intragastrically administered OST(10mg/kg)once a day for 14 days consecutively from anesthesia.Morris water maze test was used to observe rats’spatial learning and memory ability,and Western blot was used to detect the protein expression of inflammatory cytokines interleukin(IL)-1α and tumor necrosis factor(TNF)-α.Results The results of Morris water maze showed that compared with blank group,the memory ability of rats in model group decreased,and the escape latency was significantly prolonged;Compared with model group,the memory ability of rats in treatment group was improved,and the escape latency was significantly shortened.Western blot analysis revealed that IL-1β and TNF-α protein expression was increased in the hippocampus of rats in model group compared with rats in blank group,and the above protein expression was decreased in the hippocampus of rats in treatment group after intragastric treatment with OST.Conclusion OST can improve cognitive impairment induced by propofol in anesthetized rats,and the mechanism may be related to the decrease of the expression of IL-1β and TNF-α in hippocampus.

关 键 词：蛇床子素 丙泊酚 大鼠 认知功能障碍 

分 类 号：R332[医药卫生—人体生理学]