多指标正交试验法优选连豆清脉合剂提取工艺  

Optimization of Extraction Technology of Liandou Qingmai Mixture by Multi- Index Orthogonal Test

作　　者：张文明 唐静月 卞振华 袁晓航[1] 陈晓伟[1] 胡敏敏[1] 张敏娟[1] ZHANG Wenming;TANG Jingyue;BIAN Zhenhua;YUAN Xiaohang;CHEN Xiaowei;HU Minmin;ZHANG Minjuan(Wuxi Hospital of Traditional Chinese Medicine,Wuxi Jiangsu 214071,China)

机构地区：[1]无锡市中医医院,江苏无锡214071

出　　处：《新中医》2023年第17期7-12,共6页New Chinese Medicine

基　　金：无锡市中医药管理局科技项目(ZYYYB12);无锡市卫健委中青年拔尖人才资助计划项目(HB2020059);江苏省研究生科研创新计划项目(KYCX20-1513)。

摘　　要：目的:优选连豆清脉合剂最佳提取工艺。方法:采用高效液相色谱法(HPLC)测定连豆清脉合剂中连翘苷、小檗碱含量,并以连翘苷、小檗碱转移率及浸膏得率为综合评价指标,L_(9)(3^(4))正交试验法确定最佳提取工艺并进行验证;采用常压浓缩、减压浓缩方法,考察对连翘苷、小檗碱含量的影响。结果:连翘苷、小檗碱分别在5.516~110.316μg/mL、4.292~85.833μg/mL内具有良好的线性关系,平均加样回收率分别为101.31%、101.97%,RSD分别为1.58%、1.76%(n=6);连豆清脉合剂加10倍量水,浸泡30 min,提取2次,每次1.5 h为最佳提取工艺,且不同浓缩方式对连翘苷、小檗碱含量无显著性影响。结论:优选的提取工艺合理可行,为连豆清脉合剂的大规模生产及研发提供科学依据。Objective:To optimize the extraction process of Liandou Qingmai Mixture.Methods:The contents of forsythiin and berberine in Liandou Qingmai Mixture were determined by high performance liquid chromatography(HPLC).The transfer rate of forsythiin and berberine and extract yield were used as comprehensive evaluation indexes.The optimal extraction process was determined by L_(9)(3^(4))orthogonal test.The effects on the contents of forsythiin and berberine were investigated by atmospheric concentration and vacuum concentration.Results:Forsythiin and berberine had a good linear relationship in 5.516-110.316μg/mL and 4.292-85.833μg/mL,respectively.The average recoveries were 101.31%and 101.97%,RSD were 1.58%and 1.76%(n=6).The optimal extraction process was to soak Liandou Qingmai Mixture with 10 times water for 30 minutes and extract twice for 1.5 hours each time,and different concentration methods had no significant effect on the contents of forsythiin and berberine.Conclusion:The optimized extraction technology is reasonable and feasible,which provides scientific basis for the large-scale production and research and development of Liandou Qingmai Mixture.

关 键 词：连豆清脉合剂 正交试验 提取工艺 浓缩工艺 连翘苷 小檗碱 

分 类 号：R283.6[医药卫生—中药学]