锰、镉单一及复合胁迫对桑树生理生化特性的影响  

Effects of single and combined stresses of Mn and Cd on the physiological and biochemical characteristics of Morus alba

作　　者：韩淑敏 高润红[1] 王爱君 杨永志[1] 冀鹏浩 张世林[1] 星星 HAN Shumin;GAO Runhong;WANG Aijun;YANG Yongzhi;JI Penghao;ZHANG Shilin;XING Xing(College of Forestry,Inner Mongolia Agricultural University,Hohhot 010010,Inner Mongolia,China;College of Science,Inner Mongolia Agricultural University,Hohhot 010010,Inner Mongolia,China;Inner Mongolia Academy of Forestry Science,Hohhot 010010,Inner Mongolia,China;Inner Mongolia Women’s Federation,Hohhot 010098,Inner Mongolia,China;Inner Mongolia Women’s Career Development Service Center,Hohhot 010098,Inner Mongolia,China)

机构地区：[1]内蒙古农业大学林学院,内蒙古呼和浩特010010 [2]内蒙古农业大学理学院,内蒙古呼和浩特010010 [3]内蒙古自治区林业科学研究院,内蒙古呼和浩特010010 [4]内蒙古自治区妇女联合会,内蒙古呼和浩特010098 [5]内蒙古自治区妇女事业发展服务中心,内蒙古呼和浩特010098

出　　处：《中南林业科技大学学报》2023年第9期164-170,198,共8页Journal of Central South University of Forestry & Technology

基　　金：内蒙古自治区自然科学基金项目(RZ2100002371)。

摘　　要：【目的】通过抗逆性胁迫试验探究桑树对单一及复合重金属土壤污染的修复治理效果,为桑树应用于重金属污染土地恢复及生态治理方面提供理论依据和实践参考。【方法】以2年生桑树苗为试验材料,采用土培试验方法设置16个重金属污染梯度,研究不同质量浓度锰(Mn)、镉(Cd)单一及复合胁迫对其叶绿素、丙二醛(MDA)、过氧化物酶(POD)及超氧化物歧化酶(SOD)等植物逆境生理生化常见指标的变化特性及影响。【结果】在重金属胁迫过程中,对于桑树叶绿素、MDA、POD、SOD等生理特性影响程度从大到小依次为Mn、Cd复合胁迫>单一Cd胁迫>单一Mn胁迫。桑树叶绿素含量随着单一及复合胁迫梯度的增加逐渐下降;桑树MDA含量随着单一及复合胁迫梯度增加逐渐上升;POD活性和SOD活性随着单一及复合胁迫表现为第一到第三梯度呈现上升第四梯度下降,在Mn胁迫水平大于25 mg/L、Cd胁迫水平大于200 mg/L时桑树的POD、SOD活性会不同程度地受到抑制且复合胁迫下其活性变化幅度更明显,对桑树的破坏作用更大。【结论】Mn、Cd单一及复合胁迫下桑树的叶绿素、MDA、POD、SOD等生理指标变化幅度对植物的影响作用为Mn、Cd复合胁迫最大,单一Cd胁迫次之,单一Mn胁迫影响最小;叶绿素与MDA变化幅度相反,POD、SOD变化幅度相似。【Objective】The purpose of this study was to explore the stress resistance of Morus alba to the remediation and treatment of single and complex heavy metal soil pollution,to provide a theoretical basis and practical references for the application of Morus alba in the restoration and ecological treatment of heavy metal polluted land.【Method】In this study,16 heavy metal pollution gradients were set up in the soil culture test to study the effects of single and combined stresses of Mn and Cd on chlorophyll,MDA,POD,SOD and other common physiological and biochemical indices of plants.【Result】The results showed that during the process of heavy metal stress,the degree of influence on chlorophyll,MDA,POD,SOD and other physiological characteristics of Morus alba was in the order of Mn and Cd combined stress>single Cd stress>single Mn stress.The chlorophyll content of Morus alba decreased gradually with the increase of stress gradient.The MDA content of mulberry increased with the increase of stress gradient.The POD activity and SOD activity increased under the treatments of the first to the third gradients and decreased under the treatment of the fourth gradients.When the Mn stress level was greater than 25 mg/L and Cd stress level was greater than 200 mg/L,the POD and SOD activities of mulberry were inhibited to varying degrees,and the range of activity changes was more obvious under combined stress,which has greater damage effects on Morus alba.【Conclusion】The changes in the chlorophyll,MDA,POD,SOD and other physiological indexes of Morus alba under single and combined Mn and Cd stresses have the greatest effect on plants,followed by single Cd stress,and single Mn stress has the least effect.The changing amplitude of chlorophyll and MDA is opposite,while the changing amplitude of POD and SOD is similar.

关 键 词：桑树 锰污染 镉污染 复合胁迫 生理指标 

分 类 号：S718.43[农业科学—林学]