盐度变化对大海马幼体生理与摄食行为的影响  

SHORT-TERM SALINITY CHANGES ON THE PHYSIOLOGY,BIOCHEMISTRY AND FEEDING BEHAVIOR OF THE JUVENILE YELLOW SEAHORSE,HIPPOCAMPUS KUDA BLEEKER

作　　者：徐永健 柏扬 潘霞 张文馨 XU Yong-Jian;BO Yang;PAN Xia;ZHANG Wen-Xin(Key Laboratory of Aquaculture Biotechnology MOE,School of Marine Sciences,Ningbo University,Ningbo 315211,China)

机构地区：[1]宁波大学海洋学院,应用海洋生物技术教育部重点实验室,宁波315211

出　　处：《水生生物学报》2023年第12期2045-2055,共11页Acta Hydrobiologica Sinica

基　　金：宁波市自然科学基金(2017A610282);教育部重点科技项目(212070);宁波大学学科开放基金(xkzsc1508)资助。

摘　　要：为探究养殖过程中盐度微梯度变化对大海马摄食和生理的影响,实验设置8个盐度梯度(17S、19S、21S、23S、25S、27S、29S和31S),以25S为对照组,通过在短期内改变大海马养殖水体中的盐度,对大海马幼体外部行为和内部生理生化遗传参数进行了测量分析。外部行为指标为摄食响应时间、进食速率和摄食量。体内生理生化指标为SOD、CAT、MDA、AKP、ACP、胰蛋白酶、LPS、AMS活性或浓度的变化及相关功能基因Sod、Hsp70、Hsp90、Gst、Pyy、Mtor、Bcl-2、Casp9、Casp3、Gadd45α、Fadsd6、Cpt1、Fas、Cyp51、Pdha1、Mdh1、Idh3b和G6pd表达变化。结果表明,在短期盐度变化的影响下,大海马幼体的摄食行为呈规律性变化,变化幅度越大,摄食反应时间越长,摄食率和摄食量越低,均表明大海马幼体的营养摄取量减少。同时,AMS活性和MDA浓度的变化与取食行为的反应时间和摄食量显著相关,Gadd45α、Idh3b、Sod、Cpt1和Pyy基因表达与取食行为参数同时密切相关。结果可作为揭示大海马在体内和体外对盐度胁迫的响应及其相互关系的几种重要的潜在组合生物标志物,用于反映盐度对大海马幼体生长的影响。而外部行为变化可以正确反映盐度过度波动对大海马幼体的危害。In order to investigate the effects of salinity changes on the growth of juvenile Hippocampus kuda during the breeding process,eight salinity gradients(17S,19S,21S,23S,25S,27S,29S and 31S) were set in this experiment.With 25S as the control group,the external behavior and internal physiological,biochemical and genetic parameters were analyzed when the salinity of breeding was changing in a short-term.External behavioral indicators include feeding response time,feeding rate,and food intake etc.were closely related to the amplitude and rate of salinity change.The internal physiological and biochemical parameters that were analyzed in this study included the activity or concentration of SOD,CAT,MDA,AKP,ACP,Trypsin,LPS and AMS.Changes in the expression of related function genes,such as SOD,Hsp70,Hsp90,Gst,Pyy,Mtor,Bcl-2,Casp9,Casp3,Gadd45α,Fadsd6,Cpt1,Fas,Cyp51,Pdha1,Mdh1,Idh3b and G6pd were also monitored.The results showed that,the feeding behavior of juvenile H.kuda was regular changes under the influence of short-term salinity changes,feeding reaction time was prolonged,feeding rate and food intake were reduced,suggesting that the juvenile's nutrition intake decreased,meanwhile,the changes of AMS activity and MDA concentration showed a significant correlation with the reaction time,food intake of feeding behavior and the gene expressions of Gadd45α,Idh3b,SOD,Cpt1 and Pyy also had a close correlation with the behavioral parameters simultaneously.The result could become an important potential combined biomarker to reveal the responses and relationship of H.kuda in vivo and in vitro under the salinity stress,which can reflect the salinity effects on the growth of juvenile seahorse.The external behavioral changes can correctly reflect the excessive fluctuation of salinity which is harmful to the juvenile seahorse.

关 键 词：盐度 摄食行为 功能基因 潜在联合生物标志物 大海马 

分 类 号：Q178.1[生物学—水生生物学]