分裂内含肽:一种高效的无痕多肽片段连接工具  

Split Intein:a Versatile Tool for Traceless Peptide Segment Ligation

作　　者：韩东阳 任宇祥 杨子毅 黄赫 郑基深 HAN Dongyang;REN Yuxiang;YANG Ziyi;HUANG He;ZHENG Jishen(Department of Chemistry,Tsinghua University,Beijing 100084,China;Department of Life Sciences and Medicine,University of Science and Technology of China,Hefei 230027,China)

机构地区：[1]清华大学化学系,北京100084 [2]中国科学技术大学生命科学与医学部,合肥230027

出　　处：《高等学校化学学报》2023年第10期44-61,共18页Chemical Journal of Chinese Universities

基　　金：国家重点研发计划项目(批准号:2019YFA0706900);国家自然科学基金(批准号:22022703,22177108);中国科学院合肥大科学中心协同创新培育基金(批准号:2022HSC-CIP013)资助。

摘　　要：分裂内含肽通过剪接反应实现多肽片段的连接,具有高效且无痕的特点,受到了广泛关注.本文基于分裂内含肽的结构特征与剪接反应过程,结合近年来关于分裂内含肽性能优化和应用研究进展进行了综合评述,揭示其作为一种日渐成熟的蛋白质工程化技术在蛋白质化学合成领域的前景,并简要分析了目前分裂内含肽工具面临的问题与挑战,并对可能的解决方案进行了展望.Split intein can efficiently ligate peptide segments via a splicing reaction in a traceless manner and therefore has attracted great attention.Based on the structural characteristics and splicing reaction process of split intein,this paper comprehensively reviewed the recent progresses on the performance optimization and expanded applications of split intein,and revealed its great potential in the field of chemical protein synthesis as an increasingly sophisticated protein engineering technology.Finally,the challenges presented in the split intein-mediated protein trans-splicing and potential solutions in the future research were briefly discussed.

关 键 词：分裂内含肽 蛋白质剪接反应 定点修饰 蛋白质化学合成 多肽片段连接 

分 类 号：O621[理学—有机化学] O629[理学—化学]