基于GFP发光机理构建非共轭胺与环烷基醇胶束荧光体系  

A Fluorescent System Constructed by Non-conjugated Amines in Cyclohexanol Micelles Based on Simulating GFP Bioluminescence Mechanism

作　　者：兰帅 张雨 杜威龙 贾丹丹 曹磊 王东军 LAN Shuai;ZHANG Yu;DU Weilong;JIA Dandan;CAO Lei;WANG Dongjun(College of Chemical Engineering,Hebei Normal University of Science and Technology,Qinhuangdao 066004,China;Analysis and Measurement Center,Hebei Normal University of Science and Technology,Qinhuangdao 066004,China)

机构地区：[1]河北科技师范学院化学工程学院,秦皇岛066004 [2]河北科技师范学院分析测试中心,秦皇岛066004

出　　处：《高等学校化学学报》2023年第10期219-226,共8页Chemical Journal of Chinese Universities

基　　金：河北省科技计划项目(批准号:No.189A7142H)资助。

摘　　要：基于模拟绿色荧光蛋白(GFP)发光机理构建了一种新型化学发光体系,在该体系中,环烷基醇形成胶束保护非共轭有机胺高效地发射荧光.实验结果表明,该体系的形成是物理过程,且胶束微环境对荧光性能有重要影响.构建的发光体系具有荧光强度高和温敏性强等特点,可以应用于细胞染色成像和温度传感等领域.该工作为制备高效非共轭发光系统提供了一种绿色、简便和低成本的新方法.A new fluorescent system was constructed based on simulating the luminescence mechanism of green fluorescent protein(GFP),in which cycloalkyl alcohols formed micelles to protect non-conjugated organic amines to fluoresce efficiently.Only two simple procedures(mixing and aging)were necessary to fabricate the fluorescent system.The results show that the formation of the system is a physical process,and the micellar microenvironment has an important effect on the fluorescence properties.The luminescent system has the characteristics of high fluorescence intensity and strong temperature sensitivity,which can be applied in the fields of cell staining imaging and temperature sensing.At the same time,this work provides a new green,simple and low-cost method for the preparation of efficient non-conjugated luminescence systems.

关 键 词：非共轭小分子胺 环烷基醇 胶束 荧光 

分 类 号：O646[理学—物理化学] O613.71[理学—化学]