木薯细菌性萎蔫病菌2个候选抗铜基因簇的克隆和分析  

Cloning and analysis of copTAB and XmeRSA gene clusters from Xanthomonas axonopodis pv.manihotis

作　　者：徐春华[1,2] 李超萍 时涛[1] 王国芬[1] 蔡吉苗[1] 李博勋[1] 黄贵修[1] XU Chunhua;LI Chaoping;SHI Tao;WANG Guofen;CAI Jimiao;LI Boxun;HUANG Guixiu(Environment and Plant Protection Institute,CATAS,Laboratory of Integrated Pest Management on Tropical Grops,Ministry of Agriculture and Rural Affairs,P.R.China,Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests,Haikou 571101,China;Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China)

机构地区：[1]中国热带农业科学院环境与植物保护研究所/农业农村部热带作物有害生物综合治理重点实验室/海南省热带农业有害生物监测与控制重点实验室,海口571101 [2]中国热带农业科学院热带生物技术研究所,海口571101

出　　处：《热带生物学报》2023年第5期499-505,共7页Journal of Tropical Biology

基　　金：海南省自然科学基金(2019RC274);国家现代木薯产业技术体系病害防控岗项目(CARS-11-HNHGX);中国热带农业科学院基本科研业务费专项资金(1630042017003);农作物病虫鼠害疫情综合防控项目(18210019)。

摘　　要：为对木薯细菌性萎蔫病菌2个候选抗铜基因簇的功能进行验证和对病原菌抗铜机理进行分析,在进一步评价不同来源菌株抗铜性水平的基础上,开展2个基因簇的克隆工作。结果表明,来自国内及密克罗尼西亚、马来西亚等国家的供试菌株对铜离子同样具有较高水平的抗性,不同国家的代表性菌株均编码有copTAB和XmeRSA两个抗铜基因簇。不同菌株的2个基因簇具有高度同源性,分析其除参与抗铜代谢外,可能还和致病等其他保守功能相关。Cassava bacterial blight caused by Xanthomonas axonopodis pv.manihotis is one of the important diseases in the world,and it is also the most serious disease in the cassava plantations of China.Some strains in other countries with their whole genomes being sequenced were found to encode two candidate copper resistance gene clusters,copTAB and XmeRSA,and part of these strains were higher in resistance to copper ion.An attempt was made to clone copper resistance gene clusters of more strains from different other countries and analyze their copper resistance level.The results showed all the tested strains from China,Micronesia,Malaysia and other countries were also higher in resistance to copper ion.All the representative strains from these countries also encoded the copTAB and XmeRSA gene clusters.The two gene clusters from these countries were highly homologous,and they were not only involved in copper-resistance metabolism,but might also be related to other conservative functions such as pathogenicity.

关 键 词：木薯细菌性萎蔫病 抗铜基因簇 copTAB XmeRSA 

分 类 号：S435.33[农业科学—农业昆虫与害虫防治]