基于多组学分析高盐饮食诱导的大鼠高血压靶器官损伤机制  

Mechanism of Target Organ Injury Induced by High-Salt Diet in Rats with Hypertension Based on Multi-Omics Analysis

作　　者：丁瑞丰 朱嗣博 周新丽[1] DING Ruifeng;ZHU Sibo;ZHOU Xinli(School of Health Science and Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China;School of Life Sciences,Fudan University,Shanghai 200438,China)

机构地区：[1]上海理工大学健康科学与工程学院,上海200093 [2]复旦大学生命科学院,上海200438

出　　处：《食品与生物技术学报》2023年第9期99-112,共14页Journal of Food Science and Biotechnology

摘　　要：通过高盐饮食的方式构建了高血压大鼠模型,基于高通量测序技术探索了高血压靶器官(心室和心房、肾皮质和髓质、结肠)的潜在损伤机制以及高盐饮食对肠道微生物群的影响。结果表明,喂食12周高盐饮食后大鼠血压显著升高,在对高血压靶器官的转录组数据分析中发现了一系列失调的差异转录因子,16S rRNA测序结果显示大鼠肠道菌群的组成发生了改变,通过功能分析发现了这种变化与微生物组代谢功能失调密切相关。通过共表达分析,进一步探讨了基因组和微生物组之间的串扰在高血压发展中的潜在机制。综上,本研究提供了在治疗高盐饮食诱导的高血压心-肾-肠损伤相关的候选关键基因和肠道微生物群,多组学分析可能是了解基因表达模式和疾病发展的关键方法。A hypertensive rat model was established using a high-salt diet.Based on high-throughput sequencing technology,the potential injury mechanism of hypertensive target organs(ventricle and atrium,renal cortex and medulla,colon)and the effect of a high-salt diet on the intestinal microflora were explored.The results showed that rats exhibited a significant increase in blood pressure after 12 weeks of a high-salt diet.A series of dysregulated differential transcription factors were found in the transcriptome data analysis of target organs affected by hypertension.16S rRNA sequencing results showed that the composition of intestinal microflora in rats was changed.The functional analysis revealed a close association between these changes and microbiome metabolic dysfunction.Co-expression analysis further explored the potential mechanisms of crosstalk between the genome and microbiome in the development of hypertension.In conclusion,this study provides candidate key genes and the intestinal microbiome associated with the treatment of high-salt diet-induced hypertension and heart-kidney-intestinal injury.Innovative multi-omics analysis may be a crucial approach to understanding gene expression patterns and disease development.

关 键 词：高盐饮食 高血压 靶器官损伤 高通量测序 肠道菌群 

分 类 号：Q939.93[生物学—微生物学] R544.1[医药卫生—心血管疾病]