电针围刺诱导小鼠乳腺癌微血管正常化的初步研究  

Preliminary Study on Microvasculature Normalization Induced by Peritumoral Electroacupuncture in Mice With Breast Cancer Xenografts

作　　者：常馨 卢涛 黄金昶 CHANG Xin;LU Tao;HUANG Jinchang(The Third Affiliated Hospital,Beijing University of Chinese Medicine,Beijing 100029,China;College of Life Sciences,Beijing University of Chinese Medicine,Beijing 100029,China;Department of Acupuncture and Minimally Invasive Oncology,The Third Affiliated Hospital,Beijing University of Chinese Medicine,Beijing 100029,China;Institute of Acupuncture and Moxibustion in Cancer Care,Beijing University of Chinese Medicine,Beijing 100029,China)

机构地区：[1]北京中医药大学第三附属医院,北京100029 [2]北京中医药大学生命科学学院,北京100029 [3]北京中医药大学第三附属医院针灸微创肿瘤科,北京100029 [4]北京中医药大学针灸肿瘤研究所,北京100029

出　　处：《四川大学学报（医学版）》2023年第5期972-977,共6页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金面上项目(No.82074545)资助。

摘　　要：目的本研究旨在探讨电针围刺诱导小鼠乳腺癌微血管正常化的作用。方法用6~8周龄雌性BALB/c小鼠构建4T1乳腺癌皮下移植瘤模型,并随机分为荷瘤组(tumor-bearing group,TG)、电针围刺组(electroacupuncture tumorbearing group,EATG)、贝伐单抗组(bevacizumab tumor-bearing group,BTG),每组18只。TG组不予干预,EATG组电针围刺30 min,BTG组腹腔注射10 mg/kg贝伐单抗。在干预前和干预后第3、5天利用免疫荧光检测肿瘤组织CD31、α-肌动蛋白(alpha smooth muscle actin,α-SMA)、缺氧诱导因子1α(hypoxia-inducible factor 1-alpha,HIF-1α)表达情况;干预后第3天用苏木精-伊红(HE)染色、扫描电镜观察肿瘤微血管形态。结果干预前各组肿瘤组织CD31、α-SMA、HIF-1α表达均无明显差异(P>0.05)。干预后第3天,EATG组、BTG组肿瘤组织CD31、HIF-1α表达水平均明显降低(P<0.01),α-SMA表达水平均明显增加(P<0.01)。干预后第5天,EATG组、BTG组肿瘤组织CD31、HIF-1α表达水平仍低于TG组(P<0.01),α-SMA表达水平高于TG组(P<0.05)。干预后第3天,HE染色各组肿瘤组织均可见微血管;扫描电镜观察显示,TG组肿瘤微血管管壁粗糙、有缺损、管腔出现明显畸形,而EATG组、BTG组肿瘤微血管管壁完整性较好,管腔未出现明显畸形。结论电针围刺可能通过降低微血管密度,提高新生血管的周细胞覆盖,诱导小鼠乳腺癌微血管正常化,从而改善肿瘤乏氧微环境。Objective To observe the effect of peritumoral electroacupuncture on the induction of vascular normalization in a mouse breast cancer model.Methods A subcutaneous graft model of breast cancer was established with 4T1 breast cancer cell line in female BALB/c mice aged 6-8 weeks.The mice were randomly assigned to three groups,a tumor-bearing group(TG),peritumoral electroacupuncture tumor-bearing group(EATG),and bevacizumab tumor-bearing group(BTG),with 18 mice in each group.The TG mice did not receive any intervention,the EATG mice received peritumoral electroacupuncture for 30 minutes,and the BTG mice were intraperitoneally injected with bevacizumab at 10mg/kg.Immunofluorescence was performed to assess the expression of CD31/alpha smooth muscle actin(α-SMA)and hypoxia-inducible factor 1-alpha(HIF-1α)in the tumor tissue at various points of time,including before intervention and 3 days and 5 days after intervention.Then,3 days after intervention,observation of morphological changes of the microvessels in the tumor tissue was performed through Hematoxylin and Eosin(HE)staining and scanning electron microscope.Results There was no significant difference in the expression of CD31,α-SMA,and HIF-1αin the tumor tissues of all groups before experimental intervention(P>0.05).On day 3 of the experimental interventions,the CD31 and HIF-1αexpression levels in the tumor tissues of the EATG and BTG mice were significantly reduced(P<0.01),whileα-SMA expression levels were significantly increased(P<0.01)in both groups.On day 5 of the experimental interventions,the CD31 and HIF-1αexpression levels in the tumor tissues of the EATG and BTG mice were still significantly lower than those in the TG mice(P<0.01),while theα-SMA expression level was significantly higher than that in the TG group(P<0.05).On day 3 of the experimental interventions,H&E staining showed visible microvessels in the tumor tissues of all 3 groups.In addition,scanning electron microscopic observation showed that the tumor microvessel walls of the TG mice w

关 键 词：电针围刺 乳腺癌 微血管正常化 内皮细胞 周细胞 

分 类 号：R245.97[医药卫生—针灸推拿学]