定量评价南方根结线虫趋性方法的建立及应用  

Establishment and application of quantitative evaluation method for the chemotaxis of Meloidogyne incognita

作　　者：魏钰洋 谢佳 王帅[1] 丁晓帆[1] 杨紫薇 白永霞 姚友植 孙然锋 WEI Yuyang;XIE Jia;WANG Shuai;DING Xiaofan;YANG Ziwei;BAI Yongxia;YAO Youzhi;SUN Ranfeng(Key Laboratory of Green Prevention and Control of Tropical Agriculture and Forestry Biological Disasters Ministry of Education,School of Plant Protection,Hainan University,Haikou 570228,China)

机构地区：[1]海南大学植物保护学院,热带农林生物灾害绿色防控教育部重点实验室,海口570228

出　　处：《农药学学报》2023年第5期1068-1075,1046,共9页Chinese Journal of Pesticide Science

基　　金：海南省自然科学基金项目(321QN178,321CXTD436,321QN177);海南大学科研启动基金项目[KYQD(ZR)-21041].

摘　　要：为了能够定量评价南方根结线虫Meloidogyne incognita 2龄幼虫的趋化性,本研究以Pluronic F-127胶体为载体,建立了一种能够借助体式显微镜相机及ImageJ灰度值统计法对根结线虫移动轨迹进行量化检测,从而判断根结线虫2龄幼虫趋性的测试方法,并用10种已报道的具有趋性活性的化合物验证了方法的可靠性。另外,采用该方法测定了40种化合物对南方根结线虫的趋性活性。研究结果表明,10μg/mL质量浓度下,22种测试化合物具有吸引活性、11种化合物具有驱避活性,其中对茴香胺和间苯三酚分别具有较强的吸引和驱避线虫活性。进一步研究表明,对茴香胺对南方根结线虫2龄幼虫具有低浓度吸引和高浓度驱避的趋性活性,而间苯三酚对该幼虫的驱避活性随浓度增高而增强。该研究为快速定量评价化合物对南方根结线虫2龄幼虫的趋性及发现高活性根结线虫驱避剂提供了一种可靠的方法。To quantitatively evaluate the chemotaxis of the second-stage juveniles(J2s)of Meloidogyne incongnita,an experimental method using stereomicroscope camera and ImageJ grayscale statistics method to detect the chemotaxis of J2s by analyzing the movement trajectory of the root-knot nematode in Pluronic F-127 gel was established,and verified the reliability of the method with 10 reported compounds to induce chemotaxis.In addition,the method was used to determine the chemotaxis of 40 compounds against M.incongnita.The results showed that 22 compounds had attractive activity and 11 compounds had repellent activity at the concentration of 10μg/mL,with p-anisidine and phloroglucinol being the most attractive and repellent to nematodes,respectively.Further studies showed that p-anisidine exhibited attraction activity at low concentrations and repellent activity against J2s of M.incongnita at high concentrations,while the repellent activity of phloroglucinol increased with higher concentrations.This study provides a reliable method for rapid and quantitative evaluation of the chemotaxis of compounds against M.incongnita and for the discovery of highly active root-knot nematode repellents.

关 键 词：定量评价 根结线虫 趋性活性 引诱剂 驱避剂 

分 类 号：S482.51[农业科学—农药学]