基于分子检测技术的番茄和茄子嫁接番茄砧木培育方法  

Cultivation Method of Tomato and Eggplant Grafted Rootstocks Based on Molecular Detection Technology

作　　者：程琳 于彩云 魏美甜 张海娟 王文涛[3] 毛瑞喜[3] 陈福东 武玉芬 韩宇睿 王雪 程斐[4] 许勇 别之龙[6] CHENG Lin;YU Caiyun;WEI Meitian;ZHANG Haijuan;WANG Wentao;MAO Ruixi;CHEN Fudong;WU Yufen;HAN Yurui;WANG Xue;CHENG Fei;XU Yong;BIE Zhilong(Shandong Shouguang Vegetable Seed Industry Group Co.,Ltd./Shandong Province Key Laboratory of Molecular Breeding of Facility Vegetables/Shandong Shouguang Vegetable Industry Group Co.,Ltd./Key Laboratory of Facility Vegetable Germplasm Innovation of Ministry of Agriculture and Rural Affairs/Shandong Vegetable Engineering Technology Research Center,Shouguang 262700,China;Zibo Digital Agriculture Rural Development Center,Zibo 255020,China;Shandong Seed Administration Station,Jinan 250131,China;Qingdao Agricultural University,Qingdao 266000,China;Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China;Huazhong Agriculture University,Wuhan 430000,China)

机构地区：[1]山东寿光蔬菜种业集团有限公司/山东省设施蔬菜分子育种重点实验室/山东省寿光蔬菜产业集团有限公司/农业农村部设施蔬菜种质创新重点实验室/山东省蔬菜工程技术研究中心,山东寿光262700 [2]淄博市数字农业农村发展中心,山东淄博255020 [3]山东省种子管理总站,山东济南250131 [4]青岛农业大学,山东青岛266000 [5]北京市农林科学院,北京100097 [6]华中农业大学,湖北武汉430000

出　　处：《蔬菜》2023年第10期19-25,共7页Vegetables

基　　金：2021年度山东省重点研发计划重大科技创新工程项目(2021LZGC017);2022年度山东省重点研发计划竞争性创新平台项目(2022CXPT011);2022年度山东省重点研发计划农业良种工程项目(2022LZGCQY003);2022年度泰山产业领军人才。

摘　　要：为了获得能够同时作为番茄和茄子嫁接的砧木,选用自主培育的多抗番茄砧木组合26个,国内优良番茄嫁接砧木品种4个和国外引进番茄嫁接砧木3个,分别进行抗病基因包括抗根结线虫的Mi1和Mi-2、抗颈腐根腐的Frl、抗枯萎病的I-2/5和抗黄萎病的Ve1基因的分子标记检测;同时还测定了砧木品种根、茎的主要生理指标,包括壮苗指数(茎粗/株高)、根系质量和下胚轴长度等。结果表明:番茄砧木嫁接试验中,番茄接穗的嫁接成活率和移栽成活率均在95.0%以上,茄子接穗的嫁接成活率和移栽成活率均在85.0%以上。筛选出的HC483、HC484、HC490、HC493、HC494、HC495、500-2、B2和Y1共9个砧木品种综合表现较为突出,而且以这些番茄砧木培育得到的种苗长势旺盛、根系发达且抗多种病虫害。In order to obtain rootstocks that can be grafted for both tomato and eggplant,26 combinations of self-cultivated multi-resistance tomato rootstocks,4 domestic excellent tomato grafted rootstocks and 3 imported tomato grafted rootstocks were selected to detect disease resistance genes respectively,such as molecular marker detection of Mi1 and Mi-2 genes against root-knot nematodes,Frl gene against root rot,I-2/5 gene against wilt and Ve1 gene against verticillium wilt.At the same time,the main physiological indexes of roots and stems were measured,including seedling strength index(stem diameter/plant height),root mass and hypocotyl length.The results showed that the survival rate of tomato scion grafting and transplanting was more than 95.0% and that of eggplant scion grafting and transplanting was more than 85.0% with tomato rootstocks.A total of 9 rootstock cultivars including HC483,HC484,HC490,HC493,HC494,HC495,500-2,B2 and Y1,showed outstanding comprehensive performance,and the seedlings cultivated from this tomato rootstocks had strong vigor,developed roots and resistance to multiple diseases and pests.

关 键 词：番茄 砧木 分子检测 嫁接 

分 类 号：S641.2[农业科学—蔬菜学]