CMIA、TPPA与TRUST检测梅毒的临床应用  

The Clinical Application of CMIA,TPPA and TRUST Tests to Detect Syphilis

作　　者：支张卓玛 尼玛顿珠[1] 扎西央宗[1] 益西措姆 詹明君[1] 巴桑央吉[1] ZHIZHANG Zhuoma;NIMA Dunzhu;ZHAXI Yangzong;YIXI Cuomu;ZHAN Mingjun;BASANG Yangji(Department of Clinical Laboratory,The People’s Hospital of Tibet Autonomous Region,Lhasa 850000,China)

机构地区：[1]西藏自治区人民医院检验科,西藏拉萨850000

出　　处：《标记免疫分析与临床》2023年第7期1217-1219,共3页Labeled Immunoassays and Clinical Medicine

摘　　要：目的通过比较化学发光微粒子免疫分析(CMIA)、梅毒颗粒凝集试验(TPPA)与甲苯胺红不加热血清试验(TRUST)3种实验室检测梅毒的方法,分析3种检测方法的侧重方向与优缺点,评价3种方法在筛查梅毒特异性抗体中的临床应用价值。方法选取2022年在西藏自治区人民医院接受梅毒检测的627例受检者作为研究对象,同时采集所有受检者3管静脉血,采集当日采用CMIA法、TPPA试验和TRUST试验进行梅毒检测。观察3种方法的检测结果,计算结果的一致性与阴阳符合率,对计算结果进行分析。结果CMIA阳性率66.83%,TPPA阳性率59.81%,TRUST阳性率21.05%;3种方法结果一致率为52.63%;CMIA法与TPPA法检测结果一致性为91.07%;TPPA法与CMIA法阳性符合率98.40%,阴性符合率80.16%,TPPA法与TRUST法阳性符合率34.93%,阴性符合率99.60%。结论CMIA法存在较多假阳性,适合应用到梅毒筛查试验;TPPA法特异性好,准确性高,适用于确诊;TRUST法灵敏度较低,适合用来检测病程和治疗效果;3种方法联合检测可以使诊断更加准确。Objective By comparing chemiluminescence microparticle immunoassay(CMIA),syphilis particle agglutination test(TPPA)and toluidine red unheated serum test(TRUST),the emphasis direction,advantages and disadvantages of these three detection methods were analyzed,and the clinical application value of these three methods in screening syphilis specific antibodies was evaluated.Methods 627 subjects who received syphilis testing in People’s Hospital of Tibet Autonomous Region in 2022 were selected as the research cohort,and three venous blood samples were collected from all subjects at the same time point.On the day of collection,CMIA method,TPPA test and TRUST test were used to detect syphilis.We then observed the detection results of these three methods,and analyzed the consistency and negative-positive coincidence rate of the calculated results.Methods 627 subjects who underwent syphilis testing in Tibet Autonomous Region People’s Hospital in 2022 were selected as research subjects the venous blood samples of these subjects were tested for syphilis using CMIA method,TPPA test,and TRUST test.The test results of these three methods were observed,and the calculated consistent results and the positive and negative coincidence rate were evaluated.Results The positive rates of CMIA,TPPA and TRUST were 66.83%,59.81%and 21.05%,respectively.The result consistent rate of that three methods was 52.63%;The consistency between that result of CMIA and TPPA was 91.07%.The positive and negative coincidence rates of TPPA and CMIA were 98.40%and 80.16%,respectively.The positive and negative coincidence rates of TPPA and TRUST were 34.93%and 99.60%,respectively.Conclusion CMIA method has a higher false positive rate,and it is suitable for syphilis screening test.TPPA method has a good specificity and accuracy,and is suitable for the diagnosis.TRUST method has a low sensitivity,and is suitable for detecting the course of disease and therapeutic effect.The combination of these three methods can make the diagnosis more accurate.

关 键 词：梅毒抗体 化学发光微粒子免疫分析(CMIA) 梅毒颗粒凝集试验(TPPA) 甲苯胺红不加热血清试验(TRUST) 

分 类 号：R392-33[医药卫生—免疫学]