免疫组织化学染色检测肺结核组织中的Ag85B  

Detection of Ag85B in pulmonary tuberculosis by immunohistochemical staining

作　　者：方诗宇 段绍琪 陈雪倩 于江 孙杰 刘凤君 FANG Shi-yu;DUAN Shao-qi;CHEN Xue-qian;YU Jiang;SUN Jie;LIU Feng-jun(Department of Infectious Diseases,Affiliated Hospital of North Sichuan Medical College,Nanchong 637000,Sichuan,China)

机构地区：[1]川北医学附属医院感染科,四川南充637000

出　　处：《川北医学院学报》2023年第9期1165-1168,共4页Journal of North Sichuan Medical College

基　　金：四川省南充市市校合作战略合作项目(20SXQT0017);四川省南充市市校科技战略合作项目(19SXHZ0055);川北医学院附属医院项目(2020ZX003)。

摘　　要：目的:探讨结核分枝杆菌分泌性抗原Ag85B在肺结核组织中的分布特点,初步分析其在肺结核病理学诊断中的应用价值。方法:收集医院病理科手术切除的肺结核组织标本19例,以非肺结核肺组织(肺癌切除标本正常组织断端20例)为阴性对照。通过免疫组化检测Ag85B的表达情况,并与抗酸染色进行对比。结果:Ag85B免疫组化阳性信号主要沉积在结核坏死灶及周围,分布与抗酸杆菌相关、范围更广。19例肺结核标本中,10例抗酸染色阳性,抗酸染色敏感度为52.63%(10/19);10例抗酸染色阳性的标本免疫组织化学染色全部阳性,9例抗酸染色阴性的标本中免疫组化阳性的标本有6例,免疫组化敏感度为84.21%(16/19)。20例阴性对照组中,3例免疫组化呈阳性,免疫组化特异度为85.00%(17/20)。结论:Ag85B免疫组化阳性信号表达范围比抗酸杆菌分布更广,较抗酸染色具有更高的敏感性,可作为结核确诊的一种补充手段。Objective:To investigate the distribution characteristics of Mycobacterium tuberculosis secretory antigen Ag85B in pulmonary tuberculosis tissue,and to preliminarily analyze its application value in the pathological diagnosis of pulmonary tuberculosis.Methods:19 cases of pulmonary tuberculosis tissue specimens were collected from the Department of Pathology,and 20 cases of normal lung tissue(normal tissue cut end of lung cancer resection specimen)was used as a negative control.The expression of Ag85B was detected by chemical staining and compared with acid-fast staining.Results:Ag85B immunohistochemical positive signals were mainly deposited in and around the necrotic foci of tuberculosis,and the distribution was related to acid-fast bacilli and had a wider range.Among the 19 tuberculosis samples,10 had positive acid-fast staining,and the sensitivity of acid-fast staining was 52.63%(10/19).Among the 10 samples with positive acid-fast staining,all the immunohistochemical staining was positive,and among the 9 samples with negative acid-fast staining,6 samples were immunohistochemically positive,and the immunohistochemical sensitivity was 84.21%(16/19).Among the 20 negative control tissues,3 had positive immunohistochemistry,and the immunohistochemical specificity was 85.00%(17/20).Conclusion:The expression range of Ag85B immunohistochemical positive signal is wider than that of acid-fast bacilli,and it has higher sensitivity than acid-fast staining,which may be used as a supplementary method for the diagnosis of tuberculosis.

关 键 词：传染病 抗原Ag85B 免疫组织化学染色 肺结核 

分 类 号：R521[医药卫生—内科学]