盐胁迫下丛枝菌根真菌和生物炭调节黑麦草碳氮代谢的路径  

The Pathway of Arbuscular Mycorrhizal Fungi and Biochar in Regulating Carbon and Nitrogen Metabolism of Ryegrass Under Salt Stress

作　　者：赵新月 孙萍[1] 陈友媛[1,2,3] 刘佳鑫 王秀海 Zhao Xinyue;Sun Ping;Chen Youyuan;Liu Jiaxin;Wang Xiuhai(College of Environmental Science and Engineering,Ocean University of China,Qingdao 266100,China;Key Laboratory of Marine Environment and Ecology,Ministry of Education,Ocean University of China,Qingdao 266100,China;Shandong Provincial Key Laboratory of Marine Environment and Geological Engineering,Ocean University of China,Qingdao 266100,China)

机构地区：[1]中国海洋大学环境科学与工程学院,山东青岛266100 [2]中国海洋大学海洋环境与生态教育部重点实验室,山东青岛266100 [3]中国海洋大学山东省海洋环境地质工程重点实验室,山东青岛266100

出　　处：《中国海洋大学学报（自然科学版）》2023年第11期121-130,共10页Periodical of Ocean University of China

基　　金：山东省自然科学基金项目(ZR2019MD033)资助。

摘　　要：丛枝菌根真菌(AMF)和生物炭能够促进盐渍化土壤中的植物生长,但是对植物碳氮代谢的影响路径还不清晰,且缺乏对该路径的量化。本文通过水培实验,探究了不同盐胁迫(NaCl浓度0、10、20)下AMF和生物炭对黑麦草碳氮代谢关键酶和中间产物的调节作用。结果表明,AMF、生物炭分别显著提高了10‰盐胁迫下植物的株高(31.8%、19.6%)和鲜重(59.9%、21.6%),且两者联合具有协同作用;对20‰盐胁迫下的促生效果不明显。10‰盐胁迫下,AMF接种增强了植物碳代谢酶活性,蔗糖磷酸合成酶和蔗糖合成酶活性分别提高了33.9%和37.3%,积累可溶性糖含量达36.9%;生物炭添加使蔗糖磷酸合成酶和蔗糖合成酶活性分别提高了44.4%和48.3%,谷氨酰胺合成酶和谷氨酸合成酶(氮代谢酶)活性分别提高了36.9%和31.2%,积累可溶性蛋白含量达59.8%。使用偏最小二乘路径分析(PLS-PM)揭示并量化了AMF和生物炭调节植物碳氮代谢的不同路径:AMF主要调节植物的碳代谢,直接上调碳代谢关键酶的活性来影响碳代谢产物的积累;生物炭不仅调控碳代谢过程,还显著影响氮代谢,通过提高氮代谢关键酶活性,促进氮代谢产物合成与分解来实现光合作用增强。这将为盐碱地中合理施用AMF菌剂和生物炭以提高植物产量提供理论指导。Arbuscular mycorrhizal fungi(AMF)and biochar can promote plant growth in saline soil,but the pathway of them in regulating plant carbon and nitrogen metabolism remains unclear and has not been quantified.This study investigated the effects of AMF and biochar on carbon and nitrogen metabolism enzymes and intermediate products of ryegrass under different salt conditions(0、10、20 NaCl)through hydroponic experiments.The results showed that AMF and biochar significantly increased plant height(31.8%and 19.6%)and fresh weight(59.9%and 21.6%)under 10 salt stress,respectively,and the combination of AMF and biochar had synergistic effect.And there were no significant effects on promoting plant growth under 20 salt stress.Under 10‰salt stress,AMF inoculation increased carbon metabolism enzyme activities of plant.Sucrose phosphate synthetase and sucrose synthase activities were increased by 33.9%and 37.3%,respectively,and soluble sugar content was increased by 36.9%.Biochar addition increased sucrose phosphate synthetase and sucrose synthase activities by 48.3%and 44.4%,respectively;glutamine synthetase and glutamate synthetase(nitrogen metabolism enzymes)activities by 36.9%and 31.2%,respectively,and soluble protein content by 59.8%.Partial least squares path modelling(PLS-PM)was applied to reveal and quantify different pathways of AMF and biochar in regulating carbon and nitrogen metabolism of plant.AMF mainly regulated plant carbon metabolism,directly up-regulating the activities of key carbon metabolism enzymes to influence the accumulation of carbon metabolic products;biochar regulated not only carbon metabolism,but also nitrogen metabolism significantly,enhancing photosynthesis through increasing key enzymes activities of nitrogen metabolism,and promoting the synthesis and decomposition of nitrogen metabolic products.This work provides theoretical guidance for the reasonable application of AMF and biochar in saline soil to improve plant yield and quality.

关 键 词：盐胁迫 丛枝菌根真菌(AMF) 生物炭 碳氮代谢 PLS-PM模型 作用路径 

分 类 号：X53[环境科学与工程—环境工程]