硒代蛋氨酸对青鳉亲本繁育和子代发育的影响  

Effects of Selenomethionine on Parent Breeding and Offspring Development of Medaka(Oryzias latipes)

作　　者：王洁 宋磊 王欢 齐澈力木格 尹晓宇 于永利[1,2] 康桂英[1] 张学富 董武[1] 杨景峰[1] WANG Jie;SONG Lei;WANG Huan;Qichelimuge;YIN Xiaoyu;YU Yongli;KANG Guiying;ZHANG Xuefu;DONG Wu;YANG Jingfeng(Inner Mongolia Key Laboratory of Toxicant Monitoring and Toxicology,College of Animal Science and Technology,Inner Mongolia Minzu University,Tongliao 028000,China;School of Life Science and Food,Inner Mongolia Minzu University,Tongliao 028000,China;Faculty of Medicine,Inner Mongolia Minzu University,Tongliao 028000,China)

机构地区：[1]内蒙古民族大学动物科技学院,内蒙古自治区毒物监控及毒理学重点实验室,通辽028000 [2]内蒙古民族大学生命科学与食品学院,通辽028000 [3]内蒙古民族大学医学院,通辽028000

出　　处：《动物营养学报》2023年第9期5919-5930,共12页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：内蒙古自治区自然科学基金项目(82060672);内蒙古民族大学研究生科研创新资助项目(NMDSS2146);内蒙古自治区自然科学基金项目(2020MS08103,2021LHMS02007);内蒙古民族大学博士科研启动基金(BS562);学科交叉创新团队建设项目(董武-2023年度);科研创新平台能力建设支持计划(薛江东-2023年度);分子生物学仪器共享与管理项目(GXKY22001)。

摘　　要：本试验旨在研究硒代蛋氨酸(Se-Met)对青鳉亲本繁育和子代发育的影响。把17条4~5月龄青鳉随机分配到4个组,每组3~5条鱼。对照组投喂基础干饲料,试验组分别投喂含2、10和50μg/g Se-Met的干饲料。试验期7 d。结果表明,与对照组相比:1)10μg/g Se-Met组卵巢硒浓度显著升高(P<0.05);2)10、50μg/g Se-Met组的接近、求偶方向和求偶圆舞发生频率显著降低(P<0.05);3)50μg/g Se-Met组的Ⅲ期卵细胞计数显著升高(P<0.05),10和50μg/g Se-Met组的精子计数显著降低(P<0.05);4)10和50μg/g Se-Met组大脑中细胞色素P45019b(CYP19b)的mRNA相对表达水平显著升高(P<0.05),50μg/gSe-Met组卵巢中细胞色素P45019a(CYP19a)、卵泡刺激素b(FSHb)、促性腺激素释放激素1b(GnRH1b)和黄体生成素b(LHb)的mRNA相对表达水平显著升高(P<0.05);5)50μg/g Se-Met组胚胎的未受精率显著升高(P<0.05),10和50μg/g Se-Met组胚胎的畸形率显著升高(P<0.05)。由此可见,过量补充Se-Met会导致青鳉的求偶行为活动降低,雄性青鳉精原细胞数量减少,雌性青鳉卵巢及卵细胞形态紊乱,以及子代未受精率和畸形率增加,提示水产鱼类饲料添加Se-Met时应注意硒的实际需求和适当的补充剂量。This experiment was conducted to study the effects of selenomethionine(Se-Met)on parent breed-ing and offspring development of medaka.Seventeen medaka during 4 to 5 months of age were randomly divid-ed into 4 groups with 3 to 5 fish in each group.The control group was fed a basal dry feed,and the experimen-tal groups were fed dry feeds which contained 2,10 and 50μg/g Se-Met,respectively.The experiment lasted for 7 days.The results showed that compared with the control group:1)the ovarian selenium concentration of 10μg/g Se-Met group was significantly increased(P<0.05);2)the frequency of occurrence of approaching,courting orientation and courting round dance of 10 and 50μg/g Se-Met groups were significantly decreased(P<0.05);3)the egg cell count in stageⅢof 50μg/g Se-Met group was significantly increased(P<0.05),and the sperm count of 10 and 50μg/g Se-Met groups was significantly decreased(P<0.05);4)the mRNA relative expression level of cytochrome P45019b(CYP19b)in brain of 10 and 50μg/g Se-Met groups was significantly increased(P<0.05),and the mRNA relative expression levels of cytochrome P45019a(CYP19a),follicle stimulating hormone b(FSHb),gonadotropin releasing hormone 1b(GnRH1b)and lute-inizing hormone b(LHb)in ovary of 50μg/g Se-Met group were significantly increased(P<0.05);5)the mbryonic non-fertilization rate of 50μg/g Se-Met group was significantly increased(P<0.05),and the mbry-onic malformation rate of 10 and 50μg/g Se-Met groups was significantly increased(P<0.05).In conclu-sion,excessive supplementation of Se-Met can lead to in the courtship behavior activity of medaka decrease,the number of spermatogonia in male medaka decrease,the ovary and egg cell morphological disorder in fe-male medaka,and the non-fertilization rate and malformation rate in offspring increase,it suggesting that the actual demand for selenium and the appropriate amount of supplementation should be paid when adding Se-Met to aquatic fish feed.

关 键 词：青鳉 硒代蛋氨酸 生殖毒性 

分 类 号：S963.73[农业科学—水产养殖]