基于pegB基因的鸡白痢沙门氏菌、鸡伤寒沙门氏菌分子鉴别方法的建立  

pegB-PCR for Differentiation of Salmonella Gallinarum Biovar Pullorum and Gallinarum

作　　者：龚建森[1] 庄林林 张笛[1] 张萍[1] 刘向萍[1] 朱静[1] 董永毅[3] 窦新红[1] GONG Jiansen;ZHUANG Linlin;ZHANG Di;ZHANG Ping;LIU Xiangping;ZHU Jing;DONG Yongyi;DOU Xinhong(Jiangsu Institute of Poultry Sciences,Yangzhou,Jiangsu 225125;College of Animal Science and Veterinary,Jiangsu Vocational College of Agriculture and Forestry,Zhenjiang,Jiangsu 212400;Jiangsu Animal Disease Prevention and Control Center,Nanjing,Jiangsu 210036)

机构地区：[1]江苏省家禽科学研究所,江苏扬州225125 [2]江苏农林职业技术学院畜牧兽医学院,江苏镇江212400 [3]江苏省动物疫病预防控制中心,江苏南京210036

出　　处：《中国家禽》2023年第10期65-69,共5页China Poultry

基　　金：江苏现代农业产业技术体系建设项目(JATS[2022]360、JATS[2022]401);江苏省农业科技自主创新项目(CX(23)3005)。

摘　　要：为建立一种能快速、准确、简便区分鸡白痢沙门氏菌和鸡伤寒沙门氏菌的方法,研究通过对鸡白痢沙门氏菌和鸡伤寒沙门氏菌pegB序列进行分析,发现存在3个单核苷酸多态性位点,基于此建立了一种特异性PCR方法用于鉴别鸡白痢沙门氏菌和鸡伤寒沙门氏菌。结果显示,该方法特异性强、耗时短,检测限为10.14 pg/反应。临床样品检测表明,该方法不仅具有良好的特异性,且操作比传统方法更为简便。该方法的建立为准确区分鸡白痢沙门氏菌和鸡伤寒沙门氏菌提供了实用技术,将在分子检测中发挥重要作用。The objective of the present study was to establish a rapid,accurate and simple method for differentiation of Sal⁃monella Pullorum and Salmonella Gallinarum.In this study,three single nucleotide polymorphism loci were found by analyzing the pegB sequence of Salmonella Pullorum and Salmonella Gallinarum.Based on this,a specific PCR method was developed for the identification of these two biovars.The results showed that the method was rapid,simple,specific,and the detection limit was 10.14 pg/reaction.Clinical sample testing showed that this method not only had good specificity,but also was more convenient to operate than traditional methods.The establishment of this method could provide a practical technique for differentiation of Sal⁃monella Pullorum and Salmonella Gallinarum,and would play an important role in molecular detection.

关 键 词：鸡白痢沙门氏菌 鸡伤寒沙门氏菌 PCR 单核苷酸多态性 

分 类 号：S855.1[农业科学—临床兽医学]